Overview

  • Product name

  • Description

    Rabbit polyclonal to NEDD4-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide within Human NEDD4-2 aa 225-275. The exact sequence is proprietary. NP_001138439.1
    Database link: Q96PU5

  • Positive control

    • WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab245522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 112 kDa.

Target

  • Function

    E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2.
  • Tissue specificity

    Ubiquitously expressed, with highest levels in prostate, pancreas and kidney.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 4 WW domains.
  • Post-translational
    modifications

    Phosphorylated by SGK1 or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation.
    Auto-ubiquitinated.
  • Cellular localization

    Cytoplasm. May be recruited to exosomes by NDFIP1.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 ubiquitin protein ligase NEDD4 like protein antibody
    • E3 ubiquitin-protein ligase NEDD4-like antibody
    • KIAA0439 antibody
    • NED4L_HUMAN antibody
    • NEDD4 2 antibody
    • NEDD4 2c antibody
    • Nedd4-2 antibody
    • NEDD4-2a antibody
    • NEDD4.2 antibody
    • NEDD4b antibody
    • NEDD4L antibody
    • NEDD4La antibody
    • NEDD4Lb antibody
    • NEDD4Lc antibody
    • NEDD4Ld antibody
    • NEDD4Le antibody
    • NEDD4Lf antibody
    • NEDD4Lg antibody
    • NEDD4Lh antibody
    • NEDL3 antibody
    • Neural precursor cell expressed developmentally down regulated 4 like E3 ubiquitin protein ligase antibody
    • Neural precursor cell expressed, developmentally down regulated 4 like antibody
    • RSP5 antibody
    • Ubiquitin protein ligase Rsp5 antibody
    see all

Images

  • All lanes : Anti-NEDD4-2 antibody (ab245522) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 112 kDa


    Exposure time: 10 seconds
  • NEDD4-2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245522 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245522 at 0.4 µg/ml.

    Lane 1: ab245522 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 3 seconds.

References

ab245522 has not yet been referenced specifically in any publications.

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