Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab95399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 112 kDa.

Target

  • Function

    E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2.
  • Tissue specificity

    Ubiquitously expressed, with highest levels in prostate, pancreas and kidney.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 4 WW domains.
  • Post-translational
    modifications

    Phosphorylated by SGK1 or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation.
    Auto-ubiquitinated.
  • Cellular localization

    Cytoplasm. May be recruited to exosomes by NDFIP1.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 ubiquitin protein ligase NEDD4 like protein antibody
    • E3 ubiquitin-protein ligase NEDD4-like antibody
    • KIAA0439 antibody
    • NED4L_HUMAN antibody
    • NEDD4 2 antibody
    • NEDD4 2c antibody
    • Nedd4-2 antibody
    • NEDD4-2a antibody
    • NEDD4.2 antibody
    • NEDD4b antibody
    • NEDD4L antibody
    • NEDD4La antibody
    • NEDD4Lb antibody
    • NEDD4Lc antibody
    • NEDD4Ld antibody
    • NEDD4Le antibody
    • NEDD4Lf antibody
    • NEDD4Lg antibody
    • NEDD4Lh antibody
    • NEDL3 antibody
    • Neural precursor cell expressed developmentally down regulated 4 like E3 ubiquitin protein ligase antibody
    • Neural precursor cell expressed, developmentally down regulated 4 like antibody
    • RSP5 antibody
    • Ubiquitin protein ligase Rsp5 antibody
    see all

References

This product has been referenced in:

  • Gleason CE  et al. mTORC2 regulates renal tubule sodium uptake by promoting ENaC activity. J Clin Invest 125:117-28 (2015). Read more (PubMed: 25415435) »
  • Caohuy H  et al. Activation of 3-phosphoinositide-dependent kinase 1 (PDK1) and serum- and glucocorticoid-induced protein kinase 1 (SGK1) by short-chain sphingolipid C4-ceramide rescues the trafficking defect of ?F508-cystic fibrosis transmembrane conductance regulator (?F508-CFTR). J Biol Chem 289:35953-68 (2014). Read more (PubMed: 25384981) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - whole (Kidney)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
50 µg
Treatment
mini-pumps with AngII for 2 weeks
Specification
Kidney
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C

Ms. Alena Cherezova

Verified customer

Submitted Feb 19 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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