Overview

  • Product name

    Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)]
    See all NEDD4-2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8269(2)] to NEDD4-2 (phospho S448)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human NEDD4-2 (phospho S448). Corresponding to residues surrounding Serine 448.
    Database link: Q96PU5

  • Positive control

    • 293T cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab168349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 110-135 kDa (predicted molecular weight: 112 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2.
    • Tissue specificity

      Ubiquitously expressed, with highest levels in prostate, pancreas and kidney.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Contains 1 C2 domain.
      Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
      Contains 4 WW domains.
    • Post-translational
      modifications

      Phosphorylated by SGK1 or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation.
      Auto-ubiquitinated.
    • Cellular localization

      Cytoplasm. May be recruited to exosomes by NDFIP1.
    • Information by UniProt
    • Database links

    • Alternative names

      • E3 ubiquitin protein ligase NEDD4 like protein antibody
      • E3 ubiquitin-protein ligase NEDD4-like antibody
      • KIAA0439 antibody
      • NED4L_HUMAN antibody
      • NEDD4 2 antibody
      • NEDD4 2c antibody
      • Nedd4-2 antibody
      • NEDD4-2a antibody
      • NEDD4.2 antibody
      • NEDD4b antibody
      • NEDD4L antibody
      • NEDD4La antibody
      • NEDD4Lb antibody
      • NEDD4Lc antibody
      • NEDD4Ld antibody
      • NEDD4Le antibody
      • NEDD4Lf antibody
      • NEDD4Lg antibody
      • NEDD4Lh antibody
      • NEDL3 antibody
      • Neural precursor cell expressed developmentally down regulated 4 like E3 ubiquitin protein ligase antibody
      • Neural precursor cell expressed, developmentally down regulated 4 like antibody
      • RSP5 antibody
      • Ubiquitin protein ligase Rsp5 antibody
      see all

    Images

    • Detection of NEDD4-2 by Western Blot of Immunprecipitate.
      293T cell lysate immunoprecipitated using ab168349 at 1/10 dilution. HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • All lanes : Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349) at 1/1000 dilution

      Lane 1 : 293T cell lysate, untreated
      Lane 2 : 293T cell lysate, treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 112 kDa
      Additional bands at: 110-135 kDa. We are unsure as to the identity of these extra bands.

    References

    This product has been referenced in:

    • Salker MS  et al. LEFTYA Activates the Epithelial Na+ Channel (ENaC) in Endometrial Cells via Serum and Glucocorticoid Inducible Kinase SGK1. Cell Physiol Biochem 39:1295-306 (2016). Read more (PubMed: 27606670) »
    See 1 Publication for this product

    Customer reviews and Q&As

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