Recombinant Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349)
![Immunoprecipitation - Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349)](https://www.abcam.com/ps/products/168/ab168349/Images/ab168349-168675-anti-nedd4-2-phospho-s448-antibody-epr82692-immunoprecipitation.jpg "Immunoprecipitation - Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8269(2)] to NEDD4-2 (phospho S448)
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)]
See all NEDD4-2 primary antibodies -
Description
Rabbit monoclonal [EPR8269(2)] to NEDD4-2 (phospho S448) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Synthetic peptide corresponding to Human NEDD4-2 (phospho S448). Corresponding to residues surrounding Serine 448.
Database link: Q96PU5 -
Positive control
- 293T cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8269(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Detects a band of approximately 110-135 kDa (predicted molecular weight: 112 kDa).
|
|
| IP |
1/10 - 1/100.
|
| Notes |
|---|
|
WB
1/1000 - 1/5000. Detects a band of approximately 110-135 kDa (predicted molecular weight: 112 kDa). |
|
IP
1/10 - 1/100. |
Application notes
Is unsuitable for ICC/IF or IHC-P.
Target
-
Function
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2. -
Tissue specificity
Ubiquitously expressed, with highest levels in prostate, pancreas and kidney. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 4 WW domains. -
Post-translational
modificationsPhosphorylated by SGK1 or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation.
Auto-ubiquitinated. -
Cellular localization
Cytoplasm. May be recruited to exosomes by NDFIP1. - Information by UniProt
-
Database links
- Entrez Gene: 23327 Human
- Entrez Gene: 83814 Mouse
- Entrez Gene: 291553 Rat
- Omim: 606384 Human
- SwissProt: Q96PU5 Human
- SwissProt: Q8CFI0 Mouse
- Unigene: 185677 Human
- Unigene: 98668 Mouse
-
Alternative names
- E3 ubiquitin protein ligase NEDD4 like protein antibody
- E3 ubiquitin-protein ligase NEDD4-like antibody
- KIAA0439 antibody
see all
Images
-
Immunoprecipitation - Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349)
Detection of NEDD4-2 by Western Blot of Immunprecipitate.
293T cell lysate immunoprecipitated using ab168349 at 1/10 dilution. HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG. -
![Western blot - Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349)](https://www.abcam.com/ps/products/168/ab168349/Images/ab168349-168672-anti-nedd4-2-phospho-s448-antibody-epr82692-western-blot.jpg)
Western blot - Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349)All lanes : Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349) at 1/1000 dilution
Lane 1 : 293T cell lysate, untreated
Lane 2 : 293T cell lysate, treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Predicted band size: 112 kDa
Additional bands at: 110-135 kDa. We are unsure as to the identity of these extra bands. -
![Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349)](https://www.abcam.com/ps/products/168/ab168349/Images/ab168349-3-benefits-of-recombinant-antibodies.png)
Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349)
Protocols
Datasheets and documents
-
Datasheet download
References (6)
ab168349 has been referenced in 6 publications.
- Kim JE et al. AMPA Receptor Antagonists Facilitate NEDD4-2-Mediated GRIA1 Ubiquitination by Regulating PP2B-ERK1/2-SGK1 Pathway in Chronic Epilepsy Rats. Biomedicines 9:N/A (2021). PubMed: 34440273
- Lü L et al. JAK3 restrains inflammatory responses and protects against periodontal disease through Wnt3a signaling. FASEB J 34:9120-9140 (2020). PubMed: 32433819
- Yang X et al. Stimulation of Epithelial Sodium Channels in Endothelial Cells by Bone Morphogenetic Protein-4 Contributes to Salt-Sensitive Hypertension in Rats. Oxid Med Cell Longev 2020:3921897 (2020). PubMed: 33194000
- Wu MM et al. Lovastatin attenuates hypertension induced by renal tubule-specific knockout of ATP-binding cassette transporter A1, by inhibiting epithelial sodium channels. Br J Pharmacol 176:3695-3711 (2019). PubMed: 31222723
- Kemp BA et al. Ghrelin-Induced Sodium Reabsorption Is Mediated by PKA and Microtubule-Dependent aENaC Translocation in Female Rats. J Endocr Soc 3:2088-2106 (2019). PubMed: 31663064
- Salker MS et al. LEFTYA Activates the Epithelial Na+ Channel (ENaC) in Endometrial Cells via Serum and Glucocorticoid Inducible Kinase SGK1. Cell Physiol Biochem 39:1295-306 (2016). PubMed: 27606670
