Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y297] to NEDD8 (Alexa Fluor® 594)
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm
Product nameAnti-NEDD8 antibody [Y297] (Alexa Fluor® 594)
See all NEDD8 primary antibodies
DescriptionRabbit monoclonal [Y297] to NEDD8 (Alexa Fluor® 594)
ConjugationAlexa Fluor® 594. Ex: 590nm, Em: 617nm
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rat
Synthetic peptide corresponding to Human NEDD8 (N terminal).
Database link: Q15843
- ICC/IF: A673 cells
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or email@example.com.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-NEDD8 antibody [Y297] (Alexa Fluor® 647) (ab200849)
- Anti-NEDD8 antibody [Y297] (Alexa Fluor® 488) (ab201249)
- Anti-NEDD8 antibody [Y297] (Alexa Fluor® 405) (ab207248)
- Anti-NEDD8 antibody [Y297] (Alexa Fluor® 555) (ab207249)
- Anti-NEDD8 antibody [Y297] (Alexa Fluor® 568) (ab207250)
- Anti-NEDD8 antibody [Y297] - BSA and Azide free (ab220816)
- Anti-NEDD8 antibody [Y297] (ab81264)
Our Abpromise guarantee covers the use of ab207247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product gave a positive signal in A673 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)
FunctionUbiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
Tissue specificityHighly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
Sequence similaritiesBelongs to the ubiquitin family.
modificationsCleavage of precursor form by UCHL3 or SENP8 is necessary for function.
Cellular localizationNucleus. Mainly nuclear.
- Information by UniProt
- FLJ43224 antibody
- MGC104393 antibody
- MGC125896 antibody
ab207247 staining NEDD8 in A673 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207247 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in A673 cells fixed with 100% methanol (5 min)
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab207247 has not yet been referenced specifically in any publications.