Anti-NEDL1 antibody (ab121265)
Key features and details
- Rabbit polyclonal to NEDL1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NEDL1 antibody
See all NEDL1 primary antibodies -
Description
Rabbit polyclonal to NEDL1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human NEDL1 aa 356-481.
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Positive control
- Human pancreas tissue; A431 cells.
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General notes
Previously labelled as HECW1.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab121265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
ICC/IF | Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
Target
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Function
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent degradation of DVL1. Also targets the mutant SOD1 protein involved in familial amyotrophic lateral sclerosis (FALS). Forms cytotoxic aggregates with DVL1, SSR3 and mutant SOD1 that lead to motor neuron death in FALS. -
Tissue specificity
Predominantly expressed in neurons of adult and fetal brain. Weakly expressed in the kidney. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 2 WW domains. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 23072 Human
- Omim: 610384 Human
- SwissProt: Q76N89 Human
- Unigene: 164453 Human
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Alternative names
- C2 and WW domain-containing protein 1 antibody
- E3 ubiquitin-protein ligase HECW1 antibody
- HECT antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEDL1 antibody (ab121265)
ab121265 at 1/25 dilution staining NEDL1 in Paraffin-embedded Human pancreas tissue by Immunohistochemistry.
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ab121265 at 4 µg/ml staining NEDL1 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
Protocols
Datasheets and documents
References (0)
ab121265 has not yet been referenced specifically in any publications.