Anti-NEDL2 antibody (ab92711)
Key features and details
- Rabbit polyclonal to NEDL2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NEDL2 antibody
See all NEDL2 primary antibodies -
Description
Rabbit polyclonal to NEDL2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide derived from an internal region of Human NEDL2.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab92711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
WB: 1/500 - 1/1000. Predicted molecular weight: 176 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73. -
Tissue specificity
Predominantly expressed in adult brain, lung and heart. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 2 WW domains. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 57520 Human
- SwissProt: Q9P2P5 Human
- Unigene: 654742 Human
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Alternative names
- C2 and WW domain-containing protein 2 antibody
- E3 ubiquitin protein ligase HECW2 antibody
- E3 ubiquitin-protein ligase HECW2 antibody
see all
Images
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Western blot - Anti-NEDL2 antibody (ab92711)All lanes : Anti-NEDL2 antibody (ab92711) at 1/500 dilution
Lane 1 : HepG2 cell extracts
Lane 2 : LOVO cell extracts
Lane 3 : LOVO cell extracts with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 176 kDa
Datasheets and documents
References (1)
ab92711 has been referenced in 1 publication.
- Qiu X et al. NEDL2 regulates enteric nervous system and kidney development in its Nedd8 ligase activity-dependent manner. Oncotarget 7:31440-53 (2016). PubMed: 27119228
