Recombinant
RabMAb

Recombinant Anti-NEGR1 antibody [EPR8713] (ab133676)

Submit a review Q&A (2)

Overview

  • Product name

    Anti-NEGR1 antibody [EPR8713]
    See all NEGR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8713] to NEGR1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human NEGR1 (UniProt ID: Q7Z3B1).

  • Positive control

    • Human fetal brain and Human hippocampus lysates

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab133676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Relevance

      NEGR1 may be involved in cell-adhesion. May function as a trans-neural growth-promoting factor in regenerative axon sprouting in the mammalian brain.

    • Cellular localization

      Cell membrane; Lipid-anchor, GPI-anchor

    • Database links

      • Alternative names

        • A kindred of IgLON antibody
        • DMML2433 antibody
        • IgLON family member 4 antibody
        • IGLON4 antibody
        • KILON antibody
        • MGC46680 antibody
        • Neuronal growth regulator 1 antibody
        • Neurotractin antibody
        • Ntra antibody
        • PRO4993 antibody
        • UNQ2433 antibody
        see all

      Images

      • Western blot - Anti-NEGR1 antibody [EPR8713] (ab133676)
        Western blot - Anti-NEGR1 antibody [EPR8713] (ab133676)
        All lanes : Anti-NEGR1 antibody [EPR8713] (ab133676) at 1/1000 dilution

        Lane 1 : Human fetal brain lysate
        Lane 2 : Human hippocampus lysates

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 39 kDa

      References

      ab133676 has not yet been referenced specifically in any publications.

      Publishing research using ab133676? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Question

      Thank you for your response. I apologize for the delay in getting back to you, but I wanted my lab to double check that the antibody staining we had was correct - and unfortunately it appears that the Negr1 antibody (ab133676, lot number GR96807-2) is not working for us in western blots. I have attached a PDF showing a western blot using the Abcam antibody side by side with a Sigma antibody. It appears that our knockdown of Negr1 reduces the levels of the protein when probed with a Sigma antibody, but when we use the Abcam antibody. These westerns were performed with the same lysates. We have done this a few times with similar results. We also in parallel conducted RT-qPCR to ensure our knockdown, and as you can see our knockdown is very efficient.

      If possible, I would appreciate as a replacement to get the Anti-MARK2 antibody [EPR8553] (ab133724) from Abcam.

      Please let me know if this is possible. Thank you very much for your help.

      Read More
      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I have forwarded your data on to our Epitomics lab to take a look and see if they can perform further QC.

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab133724.

      To check the status of the order please contact our Customer Service team and reference this number.

      Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

      I wish you the best of luck with your research.

      Read More

      Question

      We recently bought the abcam NEGR1 antibody (ab133676, lot number GR96807-2) to use for some of our experiments and have been testing it by western blot. Unfortunately the antibody does not work as stated. Using the recommended protocols on the data sheet the antibody recognizes a band that runs at the appropriate size on a SDS-PAGE gel, but the band is not NEGR1. We have confirmed this by knocking down NEGR1, as we see no change in the band by western blot using the abcam antibody. We have confirmed knockdown using two methods: qPCR analysis which shows approximately 90% knockdown of mRNA, and western blot using antibody purchased from Sigma which shows a strong reduction in the NEGR1 protein. We would be happy to share the data with you. We were hoping to receive a refund or exchange for a different antibody as stated on the Abpromise guarantee. Please let me know what kind of information you might need.

      Read More
      Answer

      Thank you for taking time to contact us. I am sorry to hear this antibody is not providing satisfactory results.

      The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been acceptable.

      Would you be able to share some of your data with us (i.e. images of the Abcam antibody alongside the Sigma antibody)? This is one of our Epitomics products and our RabMAbs are intended to be highly specific, so I'll have to forward the data to our lab to have them take a look and reconsider some of the QA or whether or not to discontinue the ab.

      I sincerely apologize for the inconvenience. I would be happy to offer you a refund or replacement. Please tell me which you would prefer and the catalogue number for any replacement.

      Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com