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Thank you for your response. I apologize for the delay in getting back to you, but I wanted my lab to double check that the antibody staining we had was correct - and unfortunately it appears that the Negr1 antibody (ab133676, lot number GR96807-2) is not working for us in western blots. I have attached a PDF showing a western blot using the Abcam antibody side by side with a Sigma antibody. It appears that our knockdown of Negr1 reduces the levels of the protein when probed with a Sigma antibody, but when we use the Abcam antibody. These westerns were performed with the same lysates. We have done this a few times with similar results. We also in parallel conducted RT-qPCR to ensure our knockdown, and as you can see our knockdown is very efficient.
If possible, I would appreciate as a replacement to get the Anti-MARK2 antibody [EPR8553] (ab133724) from Abcam.
Please let me know if this is possible. Thank you very much for your help.
Asked on Jan 29 2013
Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I have forwarded your data on to our Epitomics lab to take a look and see if they can perform further QC.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab133724.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.
Answered on Jan 29 2013