Anti-NEIL3 antibody [2627C1b] (ab58643)

Overview

  • Product name
    Anti-NEIL3 antibody [2627C1b]
    See all NEIL3 primary antibodies
  • Description
    Mouse monoclonal [2627C1b] to NEIL3
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human NEIL3.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified using protein G column chromatography from culture supernatant of hybridoma culture in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum
  • Clonality
    Monoclonal
  • Clone number
    2627C1b
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 68 kDa.

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Dot blot Use at an assay dependent concentration.

Target

  • Function
    Reports about DNA glycosylase activity are contradictory. A number of references report finding no DNA glycosylase activity and the protein lacks a proline residue at the N-terminus which functions as an active site residue in other members of the FPG family. However, the mouse ortholog has been shown to have both DNA glycosylase and AP lyase activities and PubMed:19170771 demonstrates AP lyase activity. Prefers single-stranded DNA or partially single-stranded DNA structures such as bubble and fork structures to double-stranded DNA in vitro. Displays a broad recognition spectrum, preferring FapyA and FapyG followed by 5-OHU, 5-PHC and 5-OHMH and then Tg and 8-oxoA. No activity on 8-oxoG detected.
  • Tissue specificity
    Detected in thymus and testis. Expressed at higher levels in tumor tissue than in the corresponding normal tissues except for pancreas and testis where expression is higher in normal than tumor tissue.
  • Sequence similarities
    Belongs to the FPG family.
    Contains 1 FPG-type zinc finger.
    Contains 1 RanBP2-type zinc finger.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 123 antibody
    • AI449477 antibody
    • BC034753 antibody
    • C85903 antibody
    • DNA glycosylase FPG2 antibody
    • DNA glycosylase hFPG2 antibody
    • DNA glycosylase/AP lyase Neil3 antibody
    • EC 3.2.2.- antibody
    • EC 4.2.99.18 antibody
    • Endonuclease 8-like 3 antibody
    • Endonuclease VIII-like 3 antibody
    • FGP2 antibody
    • FLJ10858 antibody
    • FPG2 antibody
    • hFPG2 antibody
    • hNEI3 antibody
    • MGC27861 antibody
    • MGC36916 antibody
    • Nei endonuclease VIII-like 3 (E. coli) antibody
    • nei endonuclease VIII-like 3 antibody
    • Nei like 3 (E. coli) antibody
    • Nei-like protein 3 antibody
    • NEI3 antibody
    • NEIL3 antibody
    • NEIL3_HUMAN antibody
    • OTTHUMP00000218810 antibody
    • RGD1310562 antibody
    see all

Images

  • Anti-NEIL3 antibody [2627C1b] (ab58643) + immunized recombinant protein

    Predicted band size: 68 kDa
    Observed band size: 38 kDa (why is the actual band size different from the predicted?)



    The observed size may not correspond to the predicted size as the recombinant immunogen was used for the test lane.

References

ab58643 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your call today and for letting us know about the trouble with these antibodies.

As we discussed, I'm sending replacement antibodies for each of these targets. A vial of ab9134 should arrive tomorrow on the order ***, and a vial...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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