Anti-NEIL3 antibody (ab110049)
Key features and details
- Rabbit polyclonal to NEIL3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NEIL3 antibody -
Description
Rabbit polyclonal to NEIL3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Human NEIL3 (internal sequence).
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Positive control
- WB: COLO, HuvEc, HepG2 and HeLa cell extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 68 kDa.
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| Notes |
|---|
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WB
1/500 - 1/1000. Predicted molecular weight: 68 kDa. |
Target
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Function
Reports about DNA glycosylase activity are contradictory. A number of references report finding no DNA glycosylase activity and the protein lacks a proline residue at the N-terminus which functions as an active site residue in other members of the FPG family. However, the mouse ortholog has been shown to have both DNA glycosylase and AP lyase activities and PubMed:19170771 demonstrates AP lyase activity. Prefers single-stranded DNA or partially single-stranded DNA structures such as bubble and fork structures to double-stranded DNA in vitro. Displays a broad recognition spectrum, preferring FapyA and FapyG followed by 5-OHU, 5-PHC and 5-OHMH and then Tg and 8-oxoA. No activity on 8-oxoG detected. -
Tissue specificity
Detected in thymus and testis. Expressed at higher levels in tumor tissue than in the corresponding normal tissues except for pancreas and testis where expression is higher in normal than tumor tissue. -
Sequence similarities
Belongs to the FPG family.
Contains 1 FPG-type zinc finger.
Contains 1 RanBP2-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 55247 Human
- Entrez Gene: 234258 Mouse
- Omim: 608934 Human
- SwissProt: Q8TAT5 Human
- SwissProt: Q8K203 Mouse
- Unigene: 405467 Human
- Unigene: 281749 Mouse
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Alternative names
- 123 antibody
- AI449477 antibody
- BC034753 antibody
see all
Images
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Western blot - Anti-NEIL3 antibody (ab110049)All lanes : Anti-NEIL3 antibody (ab110049) at 1/500 dilution
Lane 1 : COLO cell extract
Lane 2 : HuvEc cell extract
Lane 3 : HepG2 cell extract
Lane 4 : HeLa cell extract
Lane 5 : HuvEc cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 68 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab110049 has not yet been referenced specifically in any publications.