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    neil3-antibody-ab110049.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Base Excision Repair
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Anti-NEIL3 antibody (ab110049)

  • Datasheet
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Western blot - Anti-NEIL3 antibody (ab110049)

    Key features and details

    • Rabbit polyclonal to NEIL3
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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    Overview

    • Product name

      Anti-NEIL3 antibody
    • Description

      Rabbit polyclonal to NEIL3
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse
    • Immunogen

      Synthetic peptide corresponding to Human NEIL3 (internal sequence).

    • Positive control

      • WB: COLO, HuvEc, HepG2 and HeLa cell extracts.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • Base Excision Repair

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • HeLa nuclear extract lysate (ab14655)
      • Hep G2 nuclear extract lysate (ab14660)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab110049 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/500 - 1/1000. Predicted molecular weight: 68 kDa.
    Notes
    WB
    1/500 - 1/1000. Predicted molecular weight: 68 kDa.

    Target

    • Function

      Reports about DNA glycosylase activity are contradictory. A number of references report finding no DNA glycosylase activity and the protein lacks a proline residue at the N-terminus which functions as an active site residue in other members of the FPG family. However, the mouse ortholog has been shown to have both DNA glycosylase and AP lyase activities and PubMed:19170771 demonstrates AP lyase activity. Prefers single-stranded DNA or partially single-stranded DNA structures such as bubble and fork structures to double-stranded DNA in vitro. Displays a broad recognition spectrum, preferring FapyA and FapyG followed by 5-OHU, 5-PHC and 5-OHMH and then Tg and 8-oxoA. No activity on 8-oxoG detected.
    • Tissue specificity

      Detected in thymus and testis. Expressed at higher levels in tumor tissue than in the corresponding normal tissues except for pancreas and testis where expression is higher in normal than tumor tissue.
    • Sequence similarities

      Belongs to the FPG family.
      Contains 1 FPG-type zinc finger.
      Contains 1 RanBP2-type zinc finger.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q8TAT5 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 55247 Human
      • Entrez Gene: 234258 Mouse
      • Omim: 608934 Human
      • SwissProt: Q8TAT5 Human
      • SwissProt: Q8K203 Mouse
      • Unigene: 405467 Human
      • Unigene: 281749 Mouse
      • Alternative names

        • 123 antibody
        • AI449477 antibody
        • BC034753 antibody
        • C85903 antibody
        • DNA glycosylase FPG2 antibody
        • DNA glycosylase hFPG2 antibody
        • DNA glycosylase/AP lyase Neil3 antibody
        • EC 3.2.2.- antibody
        • EC 4.2.99.18 antibody
        • Endonuclease 8-like 3 antibody
        • Endonuclease VIII-like 3 antibody
        • FGP2 antibody
        • FLJ10858 antibody
        • FPG2 antibody
        • hFPG2 antibody
        • hNEI3 antibody
        • MGC27861 antibody
        • MGC36916 antibody
        • Nei endonuclease VIII-like 3 (E. coli) antibody
        • nei endonuclease VIII-like 3 antibody
        • Nei like 3 (E. coli) antibody
        • Nei-like protein 3 antibody
        • NEI3 antibody
        • NEIL3 antibody
        • NEIL3_HUMAN antibody
        • OTTHUMP00000218810 antibody
        • RGD1310562 antibody
        see all

      Images

      • Western blot - Anti-NEIL3 antibody (ab110049)
        Western blot - Anti-NEIL3 antibody (ab110049)
        All lanes : Anti-NEIL3 antibody (ab110049) at 1/500 dilution

        Lane 1 : COLO cell extract
        Lane 2 : HuvEc cell extract
        Lane 3 : HepG2 cell extract
        Lane 4 : HeLa cell extract
        Lane 5 : HuvEc cell extract with immunising peptide at 10 µg

        Lysates/proteins at 30 µg per lane.

        Predicted band size: 68 kDa

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab110049? Please let us know so that we can cite the reference in this datasheet.

      ab110049 has not yet been referenced specifically in any publications.

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