Overview

  • Product name

  • Description

    Rabbit polyclonal to NEIL3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human NEIL3 aa 291-320 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q8TAT5

  • Positive control

    • WB: HL-60 cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab174205 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 68 kDa.

Target

  • Function

    Reports about DNA glycosylase activity are contradictory. A number of references report finding no DNA glycosylase activity and the protein lacks a proline residue at the N-terminus which functions as an active site residue in other members of the FPG family. However, the mouse ortholog has been shown to have both DNA glycosylase and AP lyase activities and PubMed:19170771 demonstrates AP lyase activity. Prefers single-stranded DNA or partially single-stranded DNA structures such as bubble and fork structures to double-stranded DNA in vitro. Displays a broad recognition spectrum, preferring FapyA and FapyG followed by 5-OHU, 5-PHC and 5-OHMH and then Tg and 8-oxoA. No activity on 8-oxoG detected.
  • Tissue specificity

    Detected in thymus and testis. Expressed at higher levels in tumor tissue than in the corresponding normal tissues except for pancreas and testis where expression is higher in normal than tumor tissue.
  • Sequence similarities

    Belongs to the FPG family.
    Contains 1 FPG-type zinc finger.
    Contains 1 RanBP2-type zinc finger.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 123 antibody
    • AI449477 antibody
    • BC034753 antibody
    • C85903 antibody
    • DNA glycosylase FPG2 antibody
    • DNA glycosylase hFPG2 antibody
    • DNA glycosylase/AP lyase Neil3 antibody
    • EC 3.2.2.- antibody
    • EC 4.2.99.18 antibody
    • Endonuclease 8-like 3 antibody
    • Endonuclease VIII-like 3 antibody
    • FGP2 antibody
    • FLJ10858 antibody
    • FPG2 antibody
    • hFPG2 antibody
    • hNEI3 antibody
    • MGC27861 antibody
    • MGC36916 antibody
    • Nei endonuclease VIII-like 3 (E. coli) antibody
    • nei endonuclease VIII-like 3 antibody
    • Nei like 3 (E. coli) antibody
    • Nei-like protein 3 antibody
    • NEI3 antibody
    • NEIL3 antibody
    • NEIL3_HUMAN antibody
    • OTTHUMP00000218810 antibody
    • RGD1310562 antibody
    see all

Images

  • Anti-NEIL3 antibody (ab174205) at 1/100 dilution + HL-60 cell lysate at 35 µg

    Predicted band size: 68 kDa

References

This product has been referenced in:

  • Klattenhoff AW  et al. Loss of NEIL3 DNA glycosylase markedly increases replication associated double strand breaks and enhances sensitivity to ATR inhibitor in glioblastoma cells. Oncotarget 8:112942-112958 (2017). Read more (PubMed: 29348879) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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