Key features and details
- Rabbit polyclonal to NEIL3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NEIL3 antibody
See all NEIL3 primary antibodies
DescriptionRabbit polyclonal to NEIL3
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human NEIL3 aa 491-540. The exact sequence is proprietary.
Database link: Q8TAT5
- IHC-P: Human colon tissue. WB: COLO, HUVEC, HepG2, and HeLa cell extracts.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol
(PBS without Mg2+ and Ca2+).
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230908 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 68 kDa.|
FunctionReports about DNA glycosylase activity are contradictory. A number of references report finding no DNA glycosylase activity and the protein lacks a proline residue at the N-terminus which functions as an active site residue in other members of the FPG family. However, the mouse ortholog has been shown to have both DNA glycosylase and AP lyase activities and PubMed:19170771 demonstrates AP lyase activity. Prefers single-stranded DNA or partially single-stranded DNA structures such as bubble and fork structures to double-stranded DNA in vitro. Displays a broad recognition spectrum, preferring FapyA and FapyG followed by 5-OHU, 5-PHC and 5-OHMH and then Tg and 8-oxoA. No activity on 8-oxoG detected.
Tissue specificityDetected in thymus and testis. Expressed at higher levels in tumor tissue than in the corresponding normal tissues except for pancreas and testis where expression is higher in normal than tumor tissue.
Sequence similaritiesBelongs to the FPG family.
Contains 1 FPG-type zinc finger.
Contains 1 RanBP2-type zinc finger.
- Information by UniProt
- 123 antibody
- AI449477 antibody
- BC034753 antibody
All lanes : Anti-NEIL3 antibody (ab230908) at 1/500 dilution
Lane 1 : COLO cell extract
Lane 2 : HUVEC (human umbilical vein endothelial cell line) cell extract
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 5 : HUVEC (human umbilical vein endothelial cell line) cell extract with immunizing peptide
Predicted band size: 68 kDa
Formalin-fixed, paraffin-embedded human colon tissue stained for NEIL3 using ab230908 at 1/50 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230908 has not yet been referenced specifically in any publications.