Product nameAnti-NEIL3 antibody
See all NEIL3 primary antibodies
DescriptionRabbit polyclonal to NEIL3
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human NEIL3 aa 491-540. The exact sequence is proprietary.
Database link: Q8TAT5
- IHC-P: Human colon tissue. WB: COLO, HUVEC, HepG2, and HeLa cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol
(PBS without Mg2+ and Ca2+).
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230908 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 68 kDa.|
FunctionReports about DNA glycosylase activity are contradictory. A number of references report finding no DNA glycosylase activity and the protein lacks a proline residue at the N-terminus which functions as an active site residue in other members of the FPG family. However, the mouse ortholog has been shown to have both DNA glycosylase and AP lyase activities and PubMed:19170771 demonstrates AP lyase activity. Prefers single-stranded DNA or partially single-stranded DNA structures such as bubble and fork structures to double-stranded DNA in vitro. Displays a broad recognition spectrum, preferring FapyA and FapyG followed by 5-OHU, 5-PHC and 5-OHMH and then Tg and 8-oxoA. No activity on 8-oxoG detected.
Tissue specificityDetected in thymus and testis. Expressed at higher levels in tumor tissue than in the corresponding normal tissues except for pancreas and testis where expression is higher in normal than tumor tissue.
Sequence similaritiesBelongs to the FPG family.
Contains 1 FPG-type zinc finger.
Contains 1 RanBP2-type zinc finger.
- Information by UniProt
- 123 antibody
- AI449477 antibody
- BC034753 antibody
All lanes : Anti-NEIL3 antibody (ab230908) at 1/500 dilution
Lane 1 : COLO cell extract
Lane 2 : HUVEC (human umbilical vein endothelial cell line) cell extract
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 5 : HUVEC (human umbilical vein endothelial cell line) cell extract with immunizing peptide
Predicted band size: 68 kDa
Formalin-fixed, paraffin-embedded human colon tissue stained for NEIL3 using ab230908 at 1/50 dilution in immunohistochemical analysis.
ab230908 has not yet been referenced specifically in any publications.