The Native Neisseria gonorrhoeae antigen is produced from a homogenised lysate of the bacteria.
Product nameNeisseria gonorrhoeae whole cell lysate - total protein
DescriptionHomogenised lysate of the bacteria
Neisseria gonorrhoeae is grown on permissive agar plates in a sterile class 2 containment hood. The plates are incubated at 37°C in the presence of 5% CO2 for 3 days. When optimal growth of colonies is seen, biomass is scraped off the plate and resuspended in sterile PBS.
A saline solution of N. gonorrhoeae is disrupted with 0.5 micron diameter glass beads to break open cells and expose more antigens. The product is then inactivated by heating to 56 °C for 3 hours and 0.09% sodium azide is added as a preservative.
Inactivation: 56 degrees for 3 hours, confirmed inactive by no visible colonies on permissive plates after 5 days.
Vortex/sonicate before use to ensure a homogenous solution.
This antigen is derived from a pathogenic organism, and may be involved in a disease process, consequently exposure may have adverse health effects.
Storage instructionsShipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab213605 has not yet been referenced specifically in any publications.