Neisseria gonorrhoeae whole cell lysate - total protein (ab213605)

Overview

  • Product name

    Neisseria gonorrhoeae whole cell lysate - total protein
  • Description

    Homogenised lysate of the bacteria
  • General notes

    Neisseria gonorrhoeae is grown on permissive agar plates in a sterile class 2 containment hood. The plates are incubated at 37°C in the presence of 5% CO2 for 3 days. When optimal growth of colonies is seen, biomass is scraped off the plate and resuspended in sterile PBS.

    A saline solution of N. gonorrhoeae is disrupted with 0.5 micron diameter glass beads to break open cells and expose more antigens. The product is then inactivated by heating to 56 °C for 3 hours and 0.09% sodium azide is added as a preservative.

    Inactivation: 56 degrees for 3 hours, confirmed inactive by no visible colonies on permissive plates after 5 days.

    Vortex/sonicate before use to ensure a homogenous solution.

    This antigen is derived from a pathogenic organism, and may be involved in a disease process, consequently exposure may have adverse health effects.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Lysate notes

    The Native Neisseria gonorrhoeae antigen is produced from a homogenised lysate of the bacteria. 

References

ab213605 has not yet been referenced specifically in any publications.

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