The Native Neisseria gonorrhoeae antigen is produced from a homogenised lysate of the bacteria.
Product nameNeisseria gonorrhoeae whole cell lysate - total protein
DescriptionHomogenised lysate of the bacteria
Neisseria gonorrhoeae is grown on permissive agar plates in a sterile class 2 containment hood. The plates are incubated at 37°C in the presence of 5% CO2 for 3 days. When optimal growth of colonies is seen, biomass is scraped off the plate and resuspended in sterile PBS.
A saline solution of N. gonorrhoeae is disrupted with 0.5 micron diameter glass beads to break open cells and expose more antigens. The product is then inactivated by heating to 56 °C for 3 hours and 0.09% sodium azide is added as a preservative.
Inactivation: 56 degrees for 3 hours, confirmed inactive by no visible colonies on permissive plates after 5 days.
Vortex/sonicate before use to ensure a homogenous solution.
This antigen is derived from a pathogenic organism, and may be involved in a disease process, consequently exposure may have adverse health effects.
Storage instructionsShipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
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ab213605 has not yet been referenced specifically in any publications.