Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab186519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.|
FunctionPhosphorylates serines and threonines, but also appears to possess tyrosine kinase activity. Implicated in the control of meiosis (By similarity). Involved in cilium assembly. In response to injury that includes DNA damage, NEK1 phosphorylates VDAC1 to limit mitochondrial cell death.
Tissue specificityHigh fetal expression in the brain and kidney.
Involvement in diseaseShort-rib thoracic dysplasia 6 with or without polydactyly
Sequence similaritiesBelongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain.
Cellular localizationNucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Associated with the pericentriolar material. Localizes to centrosome during interphase and mitosis (By similarity).
- Information by UniProt
- DKFZp686D06121 antibody
- DKFZp686K12169 antibody
- kat antibody
ab186519 has not yet been referenced specifically in any publications.