Overview

  • Product name

  • Description

    Rabbit polyclonal to NEK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human NEK1 (internal sequence). The exact sequence is proprietary.
    Database link: Q96PY6

  • Positive control

    • WB: HEK-293T whole cell extracts; mouse brain tissue extract; rat brain tissue extract. ICC/IF: U-87 MG cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab229489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 143 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Phosphorylates serines and threonines, but also appears to possess tyrosine kinase activity. Implicated in the control of meiosis (By similarity). Involved in cilium assembly. In response to injury that includes DNA damage, NEK1 phosphorylates VDAC1 to limit mitochondrial cell death.
  • Tissue specificity

    High fetal expression in the brain and kidney.
  • Involvement in disease

    Short-rib thoracic dysplasia 6 with or without polydactyly
  • Sequence similarities

    Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization

    Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Associated with the pericentriolar material. Localizes to centrosome during interphase and mitosis (By similarity).
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686D06121 antibody
    • DKFZp686K12169 antibody
    • kat antibody
    • KIAA1901 antibody
    • MGC138800 antibody
    • MGC189817 antibody
    • NEK1 antibody
    • NEK1_HUMAN antibody
    • Never in mitosis A-related kinase 1 antibody
    • Never in mitosis gene A-related kinase 1 antibody
    • NIMA (never in mitosis gene a)-related kinase 1 antibody
    • NIMA-related kinase 1 antibody
    • NimA-related protein kinase 1 antibody
    • NY-REN-55 antibody
    • OTTHUMP00000219432 antibody
    • OTTHUMP00000219433 antibody
    • OTTHUMP00000219434 antibody
    • OTTHUMP00000219435 antibody
    • Protein-serine/threonine kinase antibody
    • Renal carcinoma antigen NY-REN-55 antibody
    • Serine/threonine-protein kinase Nek1 antibody
    • SRPS2 antibody
    • SRPS2A antibody
    • SRTD6 antibody
    see all

Images

  • Anti-NEK1 antibody (ab229489) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

    Developed using the ECL technique.

    Predicted band size: 143 kDa



    5% SDS-PAGE

  • All lanes : Anti-NEK1 antibody (ab229489) at 1/1000 dilution

    Lane 1 : Mouse brain tissue extract
    Lane 2 : Rat brain tissue extract

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 143 kDa



    5% SDS-PAGE

  • 4% paraformaldehyde-fixed U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells stained for NEK1 (green) using ab229489 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab229489 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab229489.
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