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Rabbit polyclonal to NEK11
Recombinant protein fragment, corresponding to an internal region within amino acids 233-484 of Human NEK11 (UniProt ID: Q8NG66).
- A549 and HeLa cell lysates; HeLa cells.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 74 kDa.
||1/100 - 1/1000.
Possible role in S-phase checkpoint mechanism.
Poorly expressed in cerebellum, trachea, lung, appendix, and uterus.
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain.
Predominantly expressed at S/G2/M phase.
Nucleus. Nucleus > nucleolus. Nuclear during interphase but moves to the polar microtubules during prometaphase and metaphase. Accumulates in the nucleolus in G1/S-arrested cells.
Information by UniProt
- 4932416N14Rik antibody
- FLJ23495 antibody
- NEK11 antibody
Western blot - Anti-NEK11 antibody (ab155220)
Anti-NEK11 antibody (ab155220) at 1/2000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 74 kDa
7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-NEK11 antibody (ab155220)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling NEK11 using ab155220 at a 1/200 dilution. The image in the lower panel was costained with Hoechst 33342.
has not yet been referenced specifically in any publications.
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