Overview

  • Product name
  • Description
    Rabbit polyclonal to NEK11
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein fragment, corresponding to an internal region within amino acids 233-484 of Human NEK11 (UniProt ID: Q8NG66).

  • Positive control
    • A549 and HeLa cell lysates; HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 74 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Possible role in S-phase checkpoint mechanism.
  • Tissue specificity
    Poorly expressed in cerebellum, trachea, lung, appendix, and uterus.
  • Sequence similarities
    Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
  • Developmental stage
    Predominantly expressed at S/G2/M phase.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localization
    Nucleus. Nucleus > nucleolus. Nuclear during interphase but moves to the polar microtubules during prometaphase and metaphase. Accumulates in the nucleolus in G1/S-arrested cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4932416N14Rik antibody
    • FLJ23495 antibody
    • NEK11 antibody
    • NEK11_HUMAN antibody
    • NEK11S/L antibody
    • Never in mitosis A-related kinase 11 antibody
    • NIMA (never in mitosis gene a) related kinase 11 antibody
    • NIMA (never in mitosis gene a)- related kinase 11 antibody
    • NIMA related kinase 11L antibody
    • NIMA related kinase 11S antibody
    • NIMA-related kinase 11 antibody
    • NimA-related protein kinase 11 antibody
    • Serine/threonine-protein kinase Nek11 antibody
    see all

Images

  • Anti-NEK11 antibody (ab155220) at 1/2000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size: 74 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling NEK11 using ab155220 at a 1/200 dilution. The image in the lower panel was costained with Hoechst 33342.

References

ab155220 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab155220.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up