Overview

  • Product name

  • Description

    Rabbit polyclonal to NEK11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human NEK11 aa 245-482.
    Database link: Q8NG66

  • Positive control

    • WB: HeLa whole cell lysate. IHC-P: Human testis tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.03% Proclin
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab235413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 74 kDa.
IHC-P 1/20 - 1/200.

Target

  • Function

    Possible role in S-phase checkpoint mechanism.
  • Tissue specificity

    Poorly expressed in cerebellum, trachea, lung, appendix, and uterus.
  • Sequence similarities

    Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
  • Developmental stage

    Predominantly expressed at S/G2/M phase.
  • Post-translational
    modifications

    Phosphorylated.
  • Cellular localization

    Nucleus. Nucleus > nucleolus. Nuclear during interphase but moves to the polar microtubules during prometaphase and metaphase. Accumulates in the nucleolus in G1/S-arrested cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4932416N14Rik antibody
    • FLJ23495 antibody
    • NEK11 antibody
    • NEK11_HUMAN antibody
    • NEK11S/L antibody
    • Never in mitosis A-related kinase 11 antibody
    • NIMA (never in mitosis gene a) related kinase 11 antibody
    • NIMA (never in mitosis gene a)- related kinase 11 antibody
    • NIMA related kinase 11L antibody
    • NIMA related kinase 11S antibody
    • NIMA-related kinase 11 antibody
    • NimA-related protein kinase 11 antibody
    • Serine/threonine-protein kinase Nek11 antibody
    see all

Images

  • Anti-NEK11 antibody (ab235413) at 1/500 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution

    Predicted band size: 74 kDa

  • Paraffin-embedded human testis tissue stained for NEK11 using ab235413 at 1/100 dilution in immunohistochemical analysis.

References

ab235413 has not yet been referenced specifically in any publications.

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