Key features and details
- Rabbit polyclonal to NEK2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NEK2 antibody
See all NEK2 primary antibodies
DescriptionRabbit polyclonal to NEK2
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human NEK2. The exact sequence is proprietary.
Database link: P51955
- IHC-P: Human ovarian carcinoma tissue. ICC/IF: HeLa cells. WB: HEK-293T whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 52 kDa.|
FunctionProtein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore. Phosphorylates SGOL1. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.
Tissue specificityIsoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types.
Sequence similaritiesBelongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain.
Developmental stageAccumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Isoform 1 and isoform 2 expression is low in the G1 phase and increases in the S/G2 phases. Isoform 1 is absent from cells arrested in the G2/M prometaphase. Isoform 2 remains present in cells arrested in the G2/M prometaphase.
modificationsIt is unsure whether Thr-170 or Ser-171 is phosphorylated.
Cellular localizationCytoplasm. Predominantly cytoplasmic; Nucleus > nucleolus. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end and Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Co-localizes with SGOL1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope.
- Information by UniProt
- HSPK 21 antibody
- HsPK21 antibody
- NEK 2 antibody
Paraffin-embedded human ovarian carcinoma tissue stained for NEK2 using ab227958 at 1/250 dilution in immunohistochemical analysis.
Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NEK2 (green) using ab227958 at 1/500 dilution in ICC/IF.
Counterstain: alpha Tubulin filaments were labeled with anti-alpha Tubulin antibody (red) at 1/2000 dilution.
Anti-NEK2 antibody (ab227958) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Predicted band size: 52 kDa
10% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227958 has been referenced in 3 publications.
- Sun C et al. ADAM17-regulated CX3CL1 expression produced by bone marrow endothelial cells promotes spinal metastasis from hepatocellular carcinoma. Int J Oncol 57:249-263 (2020). PubMed: 32319605
- Xu T et al. Targeting NEK2 impairs oncogenesis and radioresistance via inhibiting the Wnt1/ß-catenin signaling pathway in cervical cancer. J Exp Clin Cancer Res 39:183 (2020). PubMed: 32907622
- Shen H et al. Nek2B activates the wnt pathway and promotes triple-negative breast cancer chemothezrapy-resistance by stabilizing ß-catenin. J Exp Clin Cancer Res 38:243 (2019). PubMed: 31174562