Overview

  • Product name

  • Description

    Rabbit polyclonal to NEK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human NEK2. The exact sequence is proprietary.
    Database link: P51955

  • Positive control

    • IHC-P: Human ovarian carcinoma tissue. ICC/IF: HeLa cells. WB: HEK-293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab227958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.

Target

  • Function

    Protein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore. Phosphorylates SGOL1. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.
  • Tissue specificity

    Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types.
  • Sequence similarities

    Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
  • Developmental stage

    Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Isoform 1 and isoform 2 expression is low in the G1 phase and increases in the S/G2 phases. Isoform 1 is absent from cells arrested in the G2/M prometaphase. Isoform 2 remains present in cells arrested in the G2/M prometaphase.
  • Post-translational
    modifications

    It is unsure whether Thr-170 or Ser-171 is phosphorylated.
  • Cellular localization

    Cytoplasm. Predominantly cytoplasmic; Nucleus > nucleolus. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end and Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Co-localizes with SGOL1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope.
  • Information by UniProt
  • Database links

  • Alternative names

    • HSPK 21 antibody
    • HsPK21 antibody
    • NEK 2 antibody
    • NEK2 antibody
    • NEK2_HUMAN antibody
    • NEK2A antibody
    • Never in mitosis A-related kinase 2 antibody
    • NIMA (never in mitosis gene a) related kinase 2 antibody
    • NimA like protein kinase 1 antibody
    • NIMA related kinase 2 antibody
    • NimA related protein kinase 2 antibody
    • NimA-like protein kinase 1 antibody
    • NimA-related protein kinase 2 antibody
    • NLK 1 antibody
    • NLK1 antibody
    • Serine/threonine-protein kinase Nek2 antibody
    see all

Images

  • Paraffin-embedded human ovarian carcinoma tissue stained for NEK2 using ab227958 at 1/250 dilution in immunohistochemical analysis.

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NEK2 (green) using ab227958 at 1/500 dilution in ICC/IF. 

    Counterstain: alpha Tubulin filaments were labeled with anti-alpha Tubulin antibody (red) at 1/2000 dilution.

  • Anti-NEK2 antibody (ab227958) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

    Predicted band size: 52 kDa



    10% SDS-PAGE gel.

References

ab227958 has not yet been referenced specifically in any publications.

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