Product nameAnti-NEK4 antibody [EPR8462]
See all NEK4 primary antibodies
DescriptionRabbit monoclonal [EPR8462] to NEK4
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Predicted to work with: Rat
Synthetic peptide within Human NEK4 aa 1-100. The exact sequence is proprietary.
- HeLa cells and cell lysate, Y79 cell lysate.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab150438 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 95 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceNEK4 (NIMA related kinase 4) is a serine/threonine protein kinase related to the asperigillus NIMA (never in mitosis gene a) kinase. NEK4 is proposed to play a role in cell division. Highest expression in adult heart, followed by pancreas, skeletal muscle, brain, liver, kidney, lung and placenta. Present in most primary carcinomas. There are two named isoforms produced by alternative splicing.
- EC 184.108.40.206 antibody
- MGC33171 antibody
- Never in mitosis A related kinase 4 antibody
All lanes : Anti-NEK4 antibody [EPR8462] (ab150438) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Y79 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP labelled antibody at 1/2000 dilution
Predicted band size: 95 kDa
Flow cytometric analysis of permeabilized HeLa cells labelling NEK4 with ab150438 at 1/50 (red) or a rabbit IgG (negative) (green).
ab150438 has not yet been referenced specifically in any publications.