Overview

  • Product name

    Anti-NEK4 antibody [EPR8462]
    See all NEK4 primary antibodies
  • Description

    Rabbit monoclonal [EPR8462] to NEK4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human NEK4 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • HeLa cells and cell lysate, Y79 cell lysate.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8462
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150438 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 95 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Relevance

      NEK4 (NIMA related kinase 4) is a serine/threonine protein kinase related to the asperigillus NIMA (never in mitosis gene a) kinase. NEK4 is proposed to play a role in cell division. Highest expression in adult heart, followed by pancreas, skeletal muscle, brain, liver, kidney, lung and placenta. Present in most primary carcinomas. There are two named isoforms produced by alternative splicing.
    • Cellular localization

      Nuclear
    • Database links

    • Alternative names

      • EC 2.7.11.1 antibody
      • MGC33171 antibody
      • Never in mitosis A related kinase 4 antibody
      • Never in mitosis gene A related kinase 4 antibody
      • NIMA (never in mitosis gene a) related kinase 4 antibody
      • NIMA (never in mitosis gene a)-related expressed kinase 4 antibody
      • NIMA related kinase 4 antibody
      • NimA related protein kinase 4 antibody
      • NLK2 antibody
      • NRK2 antibody
      • OTTHUMP00000213582 antibody
      • Pp12301 antibody
      • Serine/threonine kinase 2 antibody
      • Serine/threonine protein kinase 2 antibody
      • Serine/threonine protein kinase Nek4 antibody
      • Serine/threonine protein kinase NRK2 antibody
      • STK2 antibody
      see all

    Images

    • All lanes : Anti-NEK4 antibody [EPR8462] (ab150438) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Y79 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP labelled antibody at 1/2000 dilution

      Predicted band size: 95 kDa

    • Flow cytometric analysis of permeabilized HeLa cells labelling NEK4 with ab150438 at 1/50 (red) or a rabbit IgG (negative) (green).

    References

    ab150438 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2OS)
    Permeabilization
    Yes - Triton X-100 0,1%
    Specification
    U2OS
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde or Methanol

    Fernanda Basei

    Verified customer

    Submitted Sep 12 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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