Product nameAnti-NEK6 antibody [EPR5283]
See all NEK6 primary antibodies
DescriptionRabbit monoclonal [EPR5283] to NEK6
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human NEK6 (UniProt ID: Q9HC98).
- HepG2, HeLa, 293T and HT1080 cell lysates
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 36 kDa.|
|IP||1/10 - 1/100.|
FunctionActivated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis. Phosphorylates KIF11 to promote mitotic spindle formation.
Tissue specificityUbiquitous, with highest expression in heart and skeletal muscle.
Sequence similaritiesBelongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Highly similar to cell cycle protein kinase CDC5/MSD2 antibody
- NEK6 antibody
- NEK6_HUMAN antibody
All lanes : Anti-NEK6 antibody [EPR5283] (ab133494) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HT1080 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 36 kDa
This product has been referenced in:
- Freixo F et al. NEK7 regulates dendrite morphogenesis in neurons via Eg5-dependent microtubule stabilization. Nat Commun 9:2330 (2018). Read more (PubMed: 29899413) »
- Choudhury AD et al. Castration Resistance in Prostate Cancer Is Mediated by the Kinase NEK6. Cancer Res 77:753-765 (2017). Read more (PubMed: 27899381) »