Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-NEK6 antibody [EPR5283] (ab133494)

Submit a review Submit a question References (4)
Western blot - Anti-NEK6 antibody [EPR5283] (ab133494)
  • Western blot - Anti-NEK6 antibody [EPR5283] (ab133494)
  • Anti-NEK6 antibody [EPR5283] (ab133494)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5283] to NEK6
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NEK6 antibody [EPR5283]
    See all NEK6 primary antibodies

  • Description

    Rabbit monoclonal [EPR5283] to NEK6

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human NEK6 (UniProt ID: Q9HC98).

  • Positive control

    • WB: HepG2, HeLa, 293T and HT1080 cell lysates

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab133494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Activated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis. Phosphorylates KIF11 to promote mitotic spindle formation.

    • Tissue specificity

      Ubiquitous, with highest expression in heart and skeletal muscle.

    • Sequence similarities

      Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
      Contains 1 protein kinase domain.

    • Post-translational
      modifications

      Autophosphorylated.

    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Highly similar to cell cycle protein kinase CDC5/MSD2 antibody
      • NEK6 antibody
      • NEK6_HUMAN antibody
      • Never in mitosis A-related kinase 6 antibody
      • NIMA (Never In Mitosis Gene A) Related Kinase 6 antibody
      • NimA related protein kinase 6 antibody
      • NimA-related protein kinase 6 antibody
      • Protein kinase SID6 1512 antibody
      • Protein kinase SID6-1512 antibody
      • Putative serine threonine protein kinase antibody
      • Serine/threonine protein kinase Nek6 antibody
      • Serine/threonine-protein kinase Nek6 antibody
      • SID6 1512 antibody
      • SID61512 antibody
      see all

    Images

    • Western blot - Anti-NEK6 antibody [EPR5283] (ab133494)
      Western blot - Anti-NEK6 antibody [EPR5283] (ab133494)
      All lanes : Anti-NEK6 antibody [EPR5283] (ab133494) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : NEK6 knockout HeLa cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 36 kDa



      Lanes 1-3: Merged signal (red and green). Green - ab133494 observed at 36 kDa. Red - loading control ab7291 observed at 50 kDa.

      ab133494 Anti-NEK6 antibody [EPR5283] was shown to specifically react with NEK6 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265910 (knockout cell lysate ab258072) was used. Wild-type and NEK6 knockout samples were subjected to SDS-PAGE. ab133494 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

       

    • Western blot - Anti-NEK6 antibody [EPR5283] (ab133494)
      Western blot - Anti-NEK6 antibody [EPR5283] (ab133494)
      All lanes : Anti-NEK6 antibody [EPR5283] (ab133494) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HT1080 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 36 kDa

    • Anti-NEK6 antibody [EPR5283] (ab133494)
      Anti-NEK6 antibody [EPR5283] (ab133494)

    References (4)

    Publishing research using ab133494? Please let us know so that we can cite the reference in this datasheet.

    ab133494 has been referenced in 4 publications.

    • Freixo F  et al. NEK7 regulates dendrite morphogenesis in neurons via Eg5-dependent microtubule stabilization. Nat Commun 9:2330 (2018). PubMed: 29899413
    • Choudhury AD  et al. Castration Resistance in Prostate Cancer Is Mediated by the Kinase NEK6. Cancer Res 77:753-765 (2017). PubMed: 27899381
    • Shi H  et al. NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component. Nat Immunol 17:250-8 (2016). WB . PubMed: 26642356
    • He Y  et al. NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux. Nature 530:354-7 (2016). PubMed: 26814970

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab133494.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com