Recombinant
Recombinant Anti-NEK6 antibody [EPR5283] (ab133494)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-NEK6 antibody [EPR5283]
See all NEK6 primary antibodies -
Description
Rabbit monoclonal [EPR5283] to NEK6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to residues in Human NEK6 (UniProt ID: Q9HC98).
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Positive control
- HepG2, HeLa, 293T and HT1080 cell lysates
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5283 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab133494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 36 kDa. | |
| IP | 1/10 - 1/100. |
Application notes
Is unsuitable for Flow Cyt.
Target
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Function
Activated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis. Phosphorylates KIF11 to promote mitotic spindle formation. -
Tissue specificity
Ubiquitous, with highest expression in heart and skeletal muscle. -
Sequence similarities
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Clone ID: BC004174 Human
- Entrez Gene: 10783 Human
- Entrez Gene: 59126 Mouse
- Entrez Gene: 360161 Rat
- IMAGE Id: 2958695 Human
- Omim: 604884 Human
- SwissProt: Q9HC98 Human
- SwissProt: Q9ES70 Mouse
see all -
Alternative names
- Highly similar to cell cycle protein kinase CDC5/MSD2 antibody
- NEK6 antibody
- NEK6_HUMAN antibody
see all
Images
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![Western blot - Anti-NEK6 antibody [EPR5283] (ab133494)](https://www.abcam.com/ps/products/133/ab133494/Images/ab133494-147154-anti-nek6-antibody-epr5283-western-blot.jpg)
Western blot - Anti-NEK6 antibody [EPR5283] (ab133494)All lanes : Anti-NEK6 antibody [EPR5283] (ab133494) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HT1080 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 36 kDa
Datasheets and documents
References
This product has been referenced in:
- Freixo F et al. NEK7 regulates dendrite morphogenesis in neurons via Eg5-dependent microtubule stabilization. Nat Commun 9:2330 (2018). Read more (PubMed: 29899413) »
- Choudhury AD et al. Castration Resistance in Prostate Cancer Is Mediated by the Kinase NEK6. Cancer Res 77:753-765 (2017). Read more (PubMed: 27899381) »
