Anti-NEK6 antibody [OTI2H7] (ab117986)
Key features and details
- Mouse monoclonal [OTI2H7] to NEK6
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-NEK6 antibody [OTI2H7]
See all NEK6 primary antibodies -
Description
Mouse monoclonal [OTI2H7] to NEK6 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human NEK6 aa 1-350.
Database link: Q9HC98 -
Positive control
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY NEK6 cDNA. IHC-P: Human breast adenocarcinoma, kidney carcinoma, ovary, ovary adenocarcinoma, thyroid carcinoma and endometrium tissues.
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General notes
Clone OTI2H7 (formerly 2H7).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab117986 was purified from mouse ascites fluids by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI2H7 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 36 kDa.
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IHC-P |
1/50.
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Notes |
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WB
1/1000. Predicted molecular weight: 36 kDa. |
IHC-P
1/50. |
Target
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Function
Activated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis. Phosphorylates KIF11 to promote mitotic spindle formation. -
Tissue specificity
Ubiquitous, with highest expression in heart and skeletal muscle. -
Sequence similarities
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Clone ID: BC004174 Human
- Entrez Gene: 10783 Human
- IMAGE Id: 2958695 Human
- Omim: 604884 Human
- SwissProt: Q9HC98 Human
- Unigene: 197071 Human
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Alternative names
- Highly similar to cell cycle protein kinase CDC5/MSD2 antibody
- NEK6 antibody
- NEK6_HUMAN antibody
see all
Images
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All lanes : Anti-NEK6 antibody [OTI2H7] (ab117986) at 1/1000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY NEK6 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 36 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203609) -
ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human breast adenocarcinoma tissue by Immunohistochemistry.
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ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human kidney carcinoma tissue by Immunohistochemistry.
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ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human ovary tissue by Immunohistochemistry.
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ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.
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ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
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ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human endometrium tissue by Immunohistochemistry.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab117986 has been referenced in 1 publication.
- Yang ZM et al. Predictive Role of NEK6 in Prognosis and Immune Infiltration in Head and Neck Squamous Cell Carcinoma. Front Endocrinol (Lausanne) 13:943686 (2022). PubMed: 35898455