Key features and details
- Mouse monoclonal [OTI2H7] to NEK6
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-NEK6 antibody [OTI2H7]
See all NEK6 primary antibodies
DescriptionMouse monoclonal [OTI2H7] to NEK6
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human NEK6 aa 1-313.
MAGQPGHMPHGGSSNNLCHTLGPVHPPDPQRHPNTLSFRCSLADFQIEKK IGRGQFSEVYKATCLLDRKTVALKKVQIFEMMDAKARQDCVKEIGLLKQL NHPNIIKYLDSFIEDNELNIVLELADAGDLSQMIKYFKKQKRLIPERTVW KYFVQLCSAVEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSE TTAAHSLVGTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGD KMNLFSLCQKIEQCDYPPLPGEHYSEKLRELVSMCICPDPHQRPDIGYVH QVAKQMHIWMSST
Database link: Q9HC98
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY NEK6 cDNA. IHC-P: Human breast adenocarcinoma, kidney carcinoma, ovary, ovary adenocarcinoma, thyroid carcinoma and endometrium tissues.
Clone OTI2H7 (formerly 2H7).
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Purification notesab117986 was purified from mouse ascites fluids by affinity chromatography.
Our Abpromise guarantee covers the use of ab117986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 36 kDa.|
FunctionActivated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis. Phosphorylates KIF11 to promote mitotic spindle formation.
Tissue specificityUbiquitous, with highest expression in heart and skeletal muscle.
Sequence similaritiesBelongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Highly similar to cell cycle protein kinase CDC5/MSD2 antibody
- NEK6 antibody
- NEK6_HUMAN antibody
All lanes : Anti-NEK6 antibody [OTI2H7] (ab117986) at 1/1000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY NEK6 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 36 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203609)
ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human breast adenocarcinoma tissue by Immunohistochemistry.
ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human kidney carcinoma tissue by Immunohistochemistry.
ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human ovary tissue by Immunohistochemistry.
ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.
ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
ab117986, at 1/50 dilution, staining NEK6 in paraffin-embedded Human endometrium tissue by Immunohistochemistry.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab117986 has not yet been referenced specifically in any publications.