Key features and details
- Rabbit polyclonal to NEK7
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NEK7 antibody
See all NEK7 primary antibodies
DescriptionRabbit polyclonal to NEK7
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Orangutan
A region between residue 1 and 50 of human NEK7 (NP_598001.1)
- HeLa and 293T cell lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab95873 was affinity purified using an epitope specific to NEK7 immobilized on solid support.
Our Abpromise guarantee covers the use of ab95873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 35 kDa.|
|IP||Use at 5-15 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Tissue specificityHighly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.
Sequence similaritiesBelongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- EC 188.8.131.52 antibody
- NEK7 antibody
- NEK7_HUMAN antibody
All lanes : Anti-NEK7 antibody (ab95873) at 0.4 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Predicted band size: 35 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling NEK7 with ab95873 at 1/1000 (1µg/ml). Detection: DAB.
Detection of NEK7 by Western Blot of Immunprecipitate. (1) ab95873 at 0.4µg/ml staining NEK7 which had been immunoprecipitated from HeLa whole cell lysate using ab95873 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane), (2) control IgG. Detection: Chemiluminescence with exposure time of 30 seconds.
All lanes : Anti-NEK7 antibody (ab95873) at 1 µg/ml
Lane 1 : IP: ab95873 at 10µg/mg lysate
Lane 2 : IP: Controle IgG at 10µg/mg lysate
ab95873 has not yet been referenced specifically in any publications.