Anti-NEK7 antibody (ab96538)

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Western blot - Anti-NEK7 antibody (ab96538)
  • Immunocytochemistry/ Immunofluorescence - Anti-NEK7 antibody (ab96538)

Key features and details

  • Rabbit polyclonal to NEK7
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-NEK7 antibody
    See all NEK7 primary antibodies

  • Description

    Rabbit polyclonal to NEK7

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 238 and 302 of Human NEK7.

  • Positive control

    • HeLa cells

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab96538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Rat
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 35 kDa.
ICC/IF (1)
1/100 - 1/200.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 35 kDa.
ICC/IF
1/100 - 1/200.

Target

  • Tissue specificity

    Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.

  • Sequence similarities

    Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • EC 2.7.11.1 antibody
      • NEK7 antibody
      • NEK7_HUMAN antibody
      • Never in mitosis A-related kinase 7 antibody
      • Never in mitosis gene a-related kinase 7 antibody
      • NIMA (never in mitosis gene a) related kinase 7 antibody
      • NIMA (never in mitosis gene a)-related expressed kinase 7 antibody
      • NimA related protein kinase 7 antibody
      • NIMA-related kinase 7 antibody
      • NimA-related protein kinase 7 antibody
      • Serine/threonine-protein kinase Nek7 antibody
      see all

    Images

    • Western blot - Anti-NEK7 antibody (ab96538)
      Western blot - Anti-NEK7 antibody (ab96538)
      Anti-NEK7 antibody (ab96538) at 1/500 dilution + HeLa S3 whole cell lysate at 30 µg

      Predicted band size: 35 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-NEK7 antibody (ab96538)
      Immunocytochemistry/ Immunofluorescence - Anti-NEK7 antibody (ab96538)
      Top panel: ab96538, at 1/200 dilution, staining paraformaldehyde-fixed HeLa cells by immunofluorescence.
      Bottom panel: merged with DNA probe.

    References (1)

    Publishing research using ab96538? Please let us know so that we can cite the reference in this datasheet.

    ab96538 has been referenced in 1 publication.

    • de Souza EE  et al. Characterization of the human NEK7 interactome suggests catalytic and regulatory properties distinct from those of NEK6. J Proteome Res 13:4074-90 (2014). PubMed: 25093993

    Customer reviews and Q&As

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-NEK7 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.3% TritonX-100
    Specification
    HeLa
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

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    Verified customer

    Submitted Nov 26 2018

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