Anti-NEK7 antibody (ab96538)

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Overview

  • Product name

    Anti-NEK7 antibody
    See all NEK7 primary antibodies

  • Description

    Rabbit polyclonal to NEK7

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 238 and 302 of Human NEK7.

  • Positive control

    • HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab96538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 35 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Tissue specificity

    Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.

  • Sequence similarities

    Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • EC 2.7.11.1 antibody
      • NEK7 antibody
      • NEK7_HUMAN antibody
      • Never in mitosis A-related kinase 7 antibody
      • Never in mitosis gene a-related kinase 7 antibody
      • NIMA (never in mitosis gene a) related kinase 7 antibody
      • NIMA (never in mitosis gene a)-related expressed kinase 7 antibody
      • NimA related protein kinase 7 antibody
      • NIMA-related kinase 7 antibody
      • NimA-related protein kinase 7 antibody
      • Serine/threonine-protein kinase Nek7 antibody
      see all

    Images

    • Western blot - Anti-NEK7 antibody (ab96538)
      Western blot - Anti-NEK7 antibody (ab96538)
      Anti-NEK7 antibody (ab96538) at 1/500 dilution + HeLa S3 whole cell lysate at 30 µg

      Predicted band size: 35 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-NEK7 antibody (ab96538)
      Immunocytochemistry/ Immunofluorescence - Anti-NEK7 antibody (ab96538)
      Top panel: ab96538, at 1/200 dilution, staining paraformaldehyde-fixed HeLa cells by immunofluorescence.
      Bottom panel: merged with DNA probe.

    References

    This product has been referenced in:

    • de Souza EE  et al. Characterization of the human NEK7 interactome suggests catalytic and regulatory properties distinct from those of NEK6. J Proteome Res 13:4074-90 (2014). Read more (PubMed: 25093993) »
    See 1 Publication for this product

    Publishing research using ab96538? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-NEK7 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.3% TritonX-100
    Specification
    HeLa
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

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    Verified customer

    Submitted Nov 26 2018

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