Anti-NEK7 antibody (ab96538)
Key features and details
- Rabbit polyclonal to NEK7
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NEK7 antibody
See all NEK7 primary antibodies -
Description
Rabbit polyclonal to NEK7 -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC/IF HumanWB Human -
Immunogen
Synthetic peptide corresponding to a region within amino acids 238 and 302 of Human NEK7.
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Positive control
- HeLa cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC/IF |
Human
|
WB |
Human
|
All applications |
Rat
|
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 35 kDa.
|
|
ICC/IF | (1) |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 35 kDa. |
ICC/IF
1/100 - 1/200. |
Target
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Tissue specificity
Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine. -
Sequence similarities
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 140609 Human
- Entrez Gene: 360850 Rat
- Omim: 606848 Human
- SwissProt: Q8TDX7 Human
- SwissProt: D3ZBE5 Rat
- Unigene: 24119 Human
- Unigene: 723303 Human
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Alternative names
- EC 2.7.11.1 antibody
- NEK7 antibody
- NEK7_HUMAN antibody
see all
Images
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Anti-NEK7 antibody (ab96538) at 1/500 dilution + HeLa S3 whole cell lysate at 30 µg
Predicted band size: 35 kDa -
Top panel: ab96538, at 1/200 dilution, staining paraformaldehyde-fixed HeLa cells by immunofluorescence.
Bottom panel: merged with DNA probe.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab96538 has been referenced in 1 publication.
- de Souza EE et al. Characterization of the human NEK7 interactome suggests catalytic and regulatory properties distinct from those of NEK6. J Proteome Res 13:4074-90 (2014). PubMed: 25093993