Recombinant Anti-NEK7 antibody [EPR4900] (ab133514)
![Immunoprecipitation - Anti-NEK7 antibody [EPR4900] (ab133514)](https://www.abcam.com/ps/products/133/ab133514/Images/ab133514-275227-anti-nek7-antibody-epr4900-immunoprecipitation.jpg "Immunoprecipitation - Anti-NEK7 antibody [EPR4900] (ab133514)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4900] to NEK7
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-NEK7 antibody [EPR4900]
See all NEK7 primary antibodies -
Description
Rabbit monoclonal [EPR4900] to NEK7 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat, A549, C6, RAW264.7, PC12 and NIH/3T3 cell lysates. Wild-type HAP1 cell lysate. IP: NIH/3T3 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4900 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/10000. Predicted molecular weight: 35 kDa.
|
| IP |
1/10 - 1/100.
|
| Notes |
|---|
|
WB
1/10000. Predicted molecular weight: 35 kDa. |
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IP
1/10 - 1/100. |
Target
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Tissue specificity
Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine. -
Sequence similarities
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 140609 Human
- Entrez Gene: 59125 Mouse
- Entrez Gene: 360850 Rat
- Omim: 606848 Human
- SwissProt: Q8TDX7 Human
- SwissProt: Q9ES74 Mouse
- SwissProt: D3ZBE5 Rat
- Unigene: 24119 Human
see all -
Alternative names
- EC 2.7.11.1 antibody
- NEK7 antibody
- NEK7_HUMAN antibody
see all
Images
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Immunoprecipitation - Anti-NEK7 antibody [EPR4900] (ab133514)
ab133514 immunoprecipitating NEK7. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/1000.
Lane 1: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10ug
Lane 2: NIH/3T3 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab133514 in NIH/3T3 whole cell lysate -
![Western blot - Anti-NEK7 antibody [EPR4900] (ab133514)](https://www.abcam.com/ps/products/133/ab133514/Images/ab133514-275225-anti-nek7-antibody-epr4900-western-blot.jpg)
Western blot - Anti-NEK7 antibody [EPR4900] (ab133514)All lanes : Anti-NEK7 antibody [EPR4900] (ab133514) at 1/10000 dilution
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 35 kDa -
![Western blot - Anti-NEK7 antibody [EPR4900] (ab133514)](https://www.abcam.com/ps/products/133/ab133514/Images/ab133514-268423-anti-nek7-antibody-epr4900-western-blot.jpg)
Western blot - Anti-NEK7 antibody [EPR4900] (ab133514)Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: NEK7 knockout HAP1 cell lysate (40 µg)
Lane 3: A549 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab133514 observed at 35 kDa. Red - loading control, ab18058, observed at 124 kDa.ab133514 was shown to specifically react with NEK7 when NEK7 knockout samples were used. Wild-type and NEK7 knockout samples were subjected to SDS-PAGE. Ab133514 and ab18058 (loading control to Vinculin) were diluted at 1/10000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-NEK7 antibody [EPR4900] (ab133514)](https://www.abcam.com/ps/products/133/ab133514/Images/ab133514-147204-anti-nek7-antibody-epr4900-western-blot.jpg)
Western blot - Anti-NEK7 antibody [EPR4900] (ab133514)All lanes : Anti-NEK7 antibody [EPR4900] (ab133514) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : A549 cell lysate
Lane 3 : C6 cell lysate
Lane 4 : RAW264.7 cell lysate
Lane 5 : PC12 cell lysate
Lane 6 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted? -
![Anti-NEK7 antibody [EPR4900] (ab133514)](https://www.abcam.com/ps/products/133/ab133514/Images/ab133514-6-benefits-of-recombinant-antibodies.png)
Anti-NEK7 antibody [EPR4900] (ab133514)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (30)
ab133514 has been referenced in 30 publications.
- Zhang W et al. Structural analogues in herbal medicine ginseng hit a shared target to achieve cumulative bioactivity. Commun Biol 4:549 (2021). PubMed: 33972672
- Zhang T et al. ATP exposure stimulates glutathione efflux as a necessary switch for NLRP3 inflammasome activation. Redox Biol 41:101930 (2021). PubMed: 33740502
- Chang H et al. Micro-ribonucleic acid-23a-3p prevents the onset of type 2 diabetes mellitus by suppressing the activation of nucleotide-binding oligomerization-like receptor family pyrin domain containing 3 inflammatory bodies-caused pyroptosis through negatively regulating NIMA-related kinase 7. J Diabetes Investig 12:334-345 (2021). PubMed: 32881354
- Chen X et al. Identification of anti-inflammatory vesicle-like nanoparticles in honey. J Extracell Vesicles 10:e12069 (2021). PubMed: 33613874
- Lin Y et al. Gallic Acid Alleviates Gouty Arthritis by Inhibiting NLRP3 Inflammasome Activation and Pyroptosis Through Enhancing Nrf2 Signaling. Front Immunol 11:580593 (2020). PubMed: 33365024