Application
Western blot
Sample
Mouse Cell lysate - whole cell (Lysates of BMDCs)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
25000 cells
Treatment
LPS (3h, 50 ng/ml)
Specification
Lysates of BMDCs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% SMP in PBS/T
Dilution
1/10000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Detection
Detection method
ECL
Negative control
BMDCs (Nek7 ko)
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 35 kDa Non-specific: > 66 kDa kDa
Positive control
BMDCs (WT)
Additional data
Additional Notes
This antibody shows strong and Nek7 specific bands at the expected band height (around 35 kDa). The background is very low. Unspecific bands (> 66 kDa) can be detected in some, but not all samples irrespective of the genotype as shown in the blot.
BMDCs of (Lane 1) WT (Lane 2) Nek7 ko.
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Submitted Aug 24 2020