Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-NEK7 antibody [EPR4900] - BSA and Azide free (ab240065)

Overview

  • Product name

    Anti-NEK7 antibody [EPR4900] - BSA and Azide free
    See all NEK7 primary antibodies
  • Description

    Rabbit monoclonal [EPR4900] to NEK7 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NEK7 aa 1-100. The exact sequence is proprietary.

  • General notes

    Ab240065 is the carrier-free version of ab133514. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240065 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 35 kDa.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Tissue specificity

      Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.
    • Sequence similarities

      Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
      Contains 1 protein kinase domain.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • EC 2.7.11.1 antibody
      • NEK7 antibody
      • NEK7_HUMAN antibody
      • Never in mitosis A-related kinase 7 antibody
      • Never in mitosis gene a-related kinase 7 antibody
      • NIMA (never in mitosis gene a) related kinase 7 antibody
      • NIMA (never in mitosis gene a)-related expressed kinase 7 antibody
      • NimA related protein kinase 7 antibody
      • NIMA-related kinase 7 antibody
      • NimA-related protein kinase 7 antibody
      • Serine/threonine-protein kinase Nek7 antibody
      see all

    Images

    • ab133514 immunoprecipitating NEK7. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/1000.

      Lane 1: H/3T3 (Mouse embryonic fibroblast) whole cell lysate 10ug
      Lane 2: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab133514 in NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133514).

    References

    ab240065 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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