Product nameAnti-NEK7 antibody [EPR4901]
See all NEK7 primary antibodies
DescriptionRabbit monoclonal [EPR4901] to NEK7
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human NEK7 aa 250-350 (internal sequence). The exact sequence is proprietary.
- Human brain, HeLa, A549, HepG2, fetal liver, and Jurkat lysates; Jurkat cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109433 in the following tested applications.
|WB||1/1000 - 1/5000. Predicted molecular weight: 35 kDa.|
|Flow Cyt||1/100 - 1/150.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Tissue specificityHighly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.
Sequence similaritiesBelongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- EC 22.214.171.124 antibody
- NEK7 antibody
- NEK7_HUMAN antibody
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: NEK7 knockout HAP1 cell lysate (40 µg)
Lane 3: A549 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109433 observed at 35 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab109433 was shown to specifically react with NEK7 when NEK7 knockout samples were used. Wild-type and NEK7 knockout samples were subjected to SDS-PAGE. Ab109433 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-NEK7 antibody [EPR4901] (ab109433) at 1/10000 dilution
Lane 1 : Human brain lysate
Lane 2 : HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Fetal liver lysate
Lane 6 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 35 kDa
Flow cytometric analysis of NEK7 in permeabilized Jurkat cells using ab109433 at 1/100 dilution (red) or a rabbit IgG (negative) (green).