Anti-NEK8 antibody (ab169406)

Submit a review Submit a question
Western blot - Anti-NEK8 antibody (ab169406)

    Key features and details

    • Mouse polyclonal to NEK8
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-NEK8 antibody
      See all NEK8 primary antibodies

    • Description

      Mouse polyclonal to NEK8

    • Host species

      Mouse

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • Immunogen

      Full length Human NEK8 (amino acids 1-692; NP_835464.1).

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • NEK8 transfected 293T cell lysate.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab169406 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Mouse
    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Predicted molecular weight: 75 kDa.
    Notes
    WB
    Use a concentration of 1 µg/ml. Predicted molecular weight: 75 kDa.

    Target

    • Function

      Required for renal tubular integrity.

    • Tissue specificity

      Highest expression in thyroid, adrenal gland and skin. Low levels in spleen, colon and uterus. Overexpressed in breast tumors, with highest expression in infiltrating ductal carcinomas and moderate levels in mucinous adenocarcinoma.

    • Sequence similarities

      Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
      Contains 1 protein kinase domain.
      Contains 5 RCC1 repeats.

    • Cellular localization

      Cytoplasm. Predominantly cytoplasmic.
    • Information by UniProt

    • Database links

      • Alternative names

        • JCK antibody
        • NEK 8 antibody
        • NEK12A antibody
        • NEK8 antibody
        • NEK8_HUMAN antibody
        • Nephrocystin 9 antibody
        • Never in mitosis A-related kinase 8 antibody
        • NIMA (never in mitosis gene a) related kinase 8 antibody
        • NIMA related kinase 8 antibody
        • NIMA-family kinase NEK8 antibody
        • NIMA-related kinase 12a antibody
        • Nima-related protein kinase 12a antibody
        • NimA-related protein kinase 8 antibody
        • NPHP9 antibody
        • Serine/threonine-protein kinase Nek8 antibody
        see all

      Images

      • Western blot - Anti-NEK8 antibody (ab169406)
        Western blot - Anti-NEK8 antibody (ab169406)
        All lanes : Anti-NEK8 antibody (ab169406) at 1 µg/ml

        Lane 1 : NEK8 transfected 293T cell lysate
        Lane 2 : Non-transfected 293T cell lysate

        Lysates/proteins at 15 µl per lane.

        Predicted band size: 75 kDa

      References (0)

      Publishing research using ab169406? Please let us know so that we can cite the reference in this datasheet.

      ab169406 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab169406.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com