Overview

  • Product name
    Anti-NEK8 antibody - N-terminal
    See all NEK8 primary antibodies
  • Description
    Rabbit polyclonal to NEK8 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within N terminal amino acids 1-214 of Human NEK8 (UniProt ID: Q86SG6).

  • Positive control
    • 293T, A431, H1299, HeLa and Molt4 whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab153879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 75 kDa.

Target

  • Function
    Required for renal tubular integrity.
  • Tissue specificity
    Highest expression in thyroid, adrenal gland and skin. Low levels in spleen, colon and uterus. Overexpressed in breast tumors, with highest expression in infiltrating ductal carcinomas and moderate levels in mucinous adenocarcinoma.
  • Sequence similarities
    Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
    Contains 5 RCC1 repeats.
  • Cellular localization
    Cytoplasm. Predominantly cytoplasmic.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • JCK antibody
    • NEK 8 antibody
    • NEK12A antibody
    • NEK8 antibody
    • NEK8_HUMAN antibody
    • Nephrocystin 9 antibody
    • Never in mitosis A-related kinase 8 antibody
    • NIMA (never in mitosis gene a) related kinase 8 antibody
    • NIMA related kinase 8 antibody
    • NIMA-family kinase NEK8 antibody
    • NIMA-related kinase 12a antibody
    • Nima-related protein kinase 12a antibody
    • NimA-related protein kinase 8 antibody
    • NPHP9 antibody
    • Serine/threonine-protein kinase Nek8 antibody
    see all

Images

  • Western blot - Anti-NEK8 antibody - N-terminal (ab153879)
    Western blot - Anti-NEK8 antibody - N-terminal (ab153879)
    All lanes : Anti-NEK8 antibody - N-terminal (ab153879) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 75 kDa



    7.5% SDS PAGE

References

ab153879 has not yet been referenced specifically in any publications.

Publishing research using ab153879? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

There are currently no Customer reviews or Questions for ab153879.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up