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Anti-NEK8 antibody - N-terminal (ab153879)

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Western blot - Anti-NEK8 antibody - N-terminal (ab153879)

    Key features and details

    • Rabbit polyclonal to NEK8 - N-terminal
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant human NEK8 protein (ab204141)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-NEK8 antibody - N-terminal
      See all NEK8 primary antibodies
    • Description

      Rabbit polyclonal to NEK8 - N-terminal
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Cow, Zebrafish, Xenopus tropicalis
    • Immunogen

      Recombinant fragment, corresponding to a region within N terminal amino acids 1-214 of Human NEK8 (UniProt ID: Q86SG6).

    • Positive control

      • 293T, A431, H1299, HeLa and Molt4 whole cell lysates
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.00
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Cell Cycle
      • Kinases/Phosphatases
      • Other
      • Epigenetics and Nuclear Signaling
      • Cell cycle
      • Kinases/Phosphatases
      • Other
      • Cell Biology
      • Cell Cycle
      • Cell Division
      • Other cell division antibodies

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • HeLa whole cell lysate (ab150035)
      • HeLa whole cell lysate (ab29545)
      • A-431 whole cell lysate (ab30132)
      • A-431 whole cell lysate (ab7909)
      • MOLT-4 whole cell lysate (ab7912)
    • Recombinant Protein

      • Recombinant human NEK8 protein (ab204141)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab153879 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 75 kDa.
    Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 75 kDa.

    Target

    • Function

      Required for renal tubular integrity.
    • Tissue specificity

      Highest expression in thyroid, adrenal gland and skin. Low levels in spleen, colon and uterus. Overexpressed in breast tumors, with highest expression in infiltrating ductal carcinomas and moderate levels in mucinous adenocarcinoma.
    • Sequence similarities

      Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
      Contains 1 protein kinase domain.
      Contains 5 RCC1 repeats.
    • Cellular localization

      Cytoplasm. Predominantly cytoplasmic.
    • Target information above from: UniProt accession Q86SG6 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 284086 Human
      • Entrez Gene: 140859 Mouse
      • Entrez Gene: 287473 Rat
      • Entrez Gene: 171094 Zebrafish
      • Omim: 609799 Human
      • SwissProt: Q86SG6 Human
      • SwissProt: Q91ZR4 Mouse
      • SwissProt: Q90XC2 Zebrafish
      • Unigene: 448468 Human
      • Unigene: 23788 Mouse
      • Unigene: 12587 Zebrafish
      see all
    • Alternative names

      • JCK antibody
      • NEK 8 antibody
      • NEK12A antibody
      • NEK8 antibody
      • NEK8_HUMAN antibody
      • Nephrocystin 9 antibody
      • Never in mitosis A-related kinase 8 antibody
      • NIMA (never in mitosis gene a) related kinase 8 antibody
      • NIMA related kinase 8 antibody
      • NIMA-family kinase NEK8 antibody
      • NIMA-related kinase 12a antibody
      • Nima-related protein kinase 12a antibody
      • NimA-related protein kinase 8 antibody
      • NPHP9 antibody
      • Serine/threonine-protein kinase Nek8 antibody
      see all

    Images

    • Western blot - Anti-NEK8 antibody - N-terminal (ab153879)
      Western blot - Anti-NEK8 antibody - N-terminal (ab153879)
      All lanes : Anti-NEK8 antibody - N-terminal (ab153879) at 1/1000 dilution

      Lane 1 : 293T whole cell lysate
      Lane 2 : A431 whole cell lysate
      Lane 3 : H1299 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 75 kDa



      7.5% SDS PAGE

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab153879? Please let us know so that we can cite the reference in this datasheet.

    ab153879 has not yet been referenced specifically in any publications.

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