Anti-NEK8 antibody - N-terminal (ab153879)
Key features and details
- Rabbit polyclonal to NEK8 - N-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NEK8 antibody - N-terminal
See all NEK8 primary antibodies -
Description
Rabbit polyclonal to NEK8 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Zebrafish, Xenopus tropicalis
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Immunogen
Recombinant fragment, corresponding to a region within N terminal amino acids 1-214 of Human NEK8 (UniProt ID: Q86SG6).
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Positive control
- 293T, A431, H1299, HeLa and Molt4 whole cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 75 kDa.
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| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 75 kDa. |
Target
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Function
Required for renal tubular integrity. -
Tissue specificity
Highest expression in thyroid, adrenal gland and skin. Low levels in spleen, colon and uterus. Overexpressed in breast tumors, with highest expression in infiltrating ductal carcinomas and moderate levels in mucinous adenocarcinoma. -
Sequence similarities
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain.
Contains 5 RCC1 repeats. -
Cellular localization
Cytoplasm. Predominantly cytoplasmic. - Information by UniProt
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Database links
- Entrez Gene: 284086 Human
- Entrez Gene: 140859 Mouse
- Entrez Gene: 287473 Rat
- Entrez Gene: 171094 Zebrafish
- Omim: 609799 Human
- SwissProt: Q86SG6 Human
- SwissProt: Q91ZR4 Mouse
- SwissProt: Q90XC2 Zebrafish
see all -
Alternative names
- JCK antibody
- NEK 8 antibody
- NEK12A antibody
see all
Images
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Western blot - Anti-NEK8 antibody - N-terminal (ab153879)All lanes : Anti-NEK8 antibody - N-terminal (ab153879) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 75 kDa
7.5% SDS PAGE
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab153879 has not yet been referenced specifically in any publications.