Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8976(2)] to NELF
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Product nameAnti-NELF antibody [EPR8976(2)]
See all NELF primary antibodies
DescriptionRabbit monoclonal [EPR8976(2)] to NELF
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide within Human NELF aa 1-100. The exact sequence is proprietary.
Database link: Q6X4W1
- Human fetal liver and kidney, Human colon cancer, Human testis lysates; PC-3 cells
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab183022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCouples NMDA receptor signaling to the nucleus. Influences outgrowth of olfactory axons and migration of LHRH neurons.
Tissue specificityHighly expressed in adult and fetal brain, testis and kidney.
Involvement in diseaseDefects in NELF may be associated with idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.
Cellular localizationCell membrane. Nucleus. Found on the outside of the LHRH cell membrane and axons projecting from the olfactory pit and epithelium. Myristoylation is a prerequisite for extranuclear localization.
- Information by UniProt
- Nasal embryonic LHRH factor antibody
- Nasal embryonic luteinizing hormone releasing hormone factor antibody
- Nasal embryonic luteinizing hormone-releasing hormone factor antibody
Flow cytometry analysis of PC3 cells labeling NELF using ab183022 at 1/300 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.
All lanes : Anti-NELF antibody [EPR8976(2)] (ab183022) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human colon cancer lysate
Lane 4 : Human testis lysate
Lysates/proteins at 10 µg per lane.
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 60 kDa
ab183022 has not yet been referenced specifically in any publications.