Recombinant Anti-NELF-B antibody [EPR11200] (ab167401)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11200] to NELF-B
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NELF-B antibody [EPR11200]
See all NELF-B primary antibodies -
Description
Rabbit monoclonal [EPR11200] to NELF-B -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment within Human NELF-B. The exact sequence is proprietary.
Database link: Q8WX92 -
Positive control
- T47D, HeLa, BxPC-3 and HepG2 whole cell lysate (ab7900); Human colon and Human uterus tissues; BxPC-3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11200 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab167401 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 66 kDa.
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ICC/IF | (1) |
1/100 - 1/250.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 66 kDa. |
ICC/IF
1/100 - 1/250. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
NELF-B is an essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex causes transcriptional pausing and is counteracted by the P-TEFb kinase complex. It may be able to induce chromatin unfolding. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 25920 Human
- Entrez Gene: 58202 Mouse
- Entrez Gene: 311796 Rat
- Omim: 611180 Human
- SwissProt: Q8WX92 Human
- SwissProt: Q8C4Y3 Mouse
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Alternative names
- A730008L03Rik antibody
- AB041607 antibody
- AI663983 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human kidney tissue stained for NELF-B with ab167401 (1/100 dilution) in immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Formalin-fixed, paraffin-embedded human pancreas tissue stained for NELF-B with ab167401 (1/100 dilution) in immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of BxPC-3 cells labeling NELF-B with ab167401 at 1/100 dilution.
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All lanes : Anti-NELF-B antibody [EPR11200] (ab167401) at 1/1000 dilution
Lane 1 : T47D cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : BxPC-3 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 66 kDa -
Formalin-fixed, paraffin-embedded human colon tissue stained for NELF-B with ab167401 (1/100 dilution) in immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Formalin-fixed, paraffin-embedded human uterus tissue stained for NELF-B with ab167401 (1/100 dilution) in immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab167401 has been referenced in 6 publications.
- Ou J et al. Epithelial NELF guards intestinal barrier function to ameliorate colitis by maintaining junctional integrity. Mucosal Immunol 15:279-288 (2022). PubMed: 34697434
- Abe K et al. Distinct patterns of RNA polymerase II and transcriptional elongation characterize mammalian genome activation. Cell Rep 41:111865 (2022). PubMed: 36577375
- Ghouraba MH et al. Role of NELF-B in supporting epithelial-mesenchymal transition and cell proliferation during hepatocellular carcinoma progression. Oncol Lett 22:761 (2021). PubMed: 34539865
- Yu L et al. Negative elongation factor complex enables macrophage inflammatory responses by controlling anti-inflammatory gene expression. Nat Commun 11:2286 (2020). PubMed: 32385332
- Awwad SW et al. NELF-E is recruited to DNA double-strand break sites to promote transcriptional repression and repair. EMBO Rep 18:745-764 (2017). PubMed: 28336775
- El Zeneini E et al. Knockdown of COBRA1 decreases the proliferation and migration of hepatocellular carcinoma cells. Oncol Rep 37:1896-1906 (2017). PubMed: 28112367