Overview

  • Product name

    Anti-NELF-B antibody [EPR11200]
    See all NELF-B primary antibodies
  • Description

    Rabbit monoclonal [EPR11200] to NELF-B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human NELF-B. The exact sequence is proprietary.
    Database link: Q8WX92

  • Positive control

    • T47D, HeLa, BxPC-3 and HepG2 whole cell lysate (ab7900); Human colon and Human uterus tissues; BxPC-3 cells.
  • General notes

     

     

    Previously labelled as COBRA1. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11200
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab167401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 66 kDa.
ICC/IF 1/100 - 1/250.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Relevance

      NELF-B is an essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex causes transcriptional pausing and is counteracted by the P-TEFb kinase complex. It may be able to induce chromatin unfolding.
    • Cellular localization

      Nuclear
    • Database links

    • Alternative names

      • A730008L03Rik antibody
      • AB041607 antibody
      • AI663983 antibody
      • Cobra1 antibody
      • Cofactor of BRCA1 antibody
      • DKFZp586B0519 antibody
      • KIAA1182 antibody
      • Negative elongation factor B antibody
      • Negative elongation factor protein B antibody
      • Nelf b antibody
      • NELF-B antibody
      • NELFB antibody
      • RGD1307832 antibody
      • RP13-122B23.3 antibody
      • RP23-132N23.4 antibody
      see all

    Images

    • Formalin-fixed, paraffin-embedded human kidney tissue stained for NELF-B with ab167401 (1/100 dilution) in immunohistochemical analysis.

    • Formalin-fixed, paraffin-embedded human pancreas tissue stained for NELF-B with ab167401 (1/100 dilution) in immunohistochemical analysis.

    • Immunofluorescent analysis of BxPC-3 cells labeling NELF-B with ab167401 at 1/100 dilution.

    • All lanes : Anti-NELF-B antibody [EPR11200] (ab167401) at 1/1000 dilution

      Lane 1 : T47D cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : BxPC-3 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 66 kDa

    • Formalin-fixed, paraffin-embedded human colon tissue stained for NELF-B with ab167401 (1/100 dilution) in immunohistochemical analysis.

    • Formalin-fixed, paraffin-embedded human uterus tissue stained for NELF-B with ab167401 (1/100 dilution) in immunohistochemical analysis.

    References

    This product has been referenced in:

    • Awwad SW  et al. NELF-E is recruited to DNA double-strand break sites to promote transcriptional repression and repair. EMBO Rep 18:745-764 (2017). Read more (PubMed: 28336775) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (mouse embryonic stem cell line)
    Permeabilization
    Yes - 0.5% triton
    Specification
    mouse embryonic stem cell line
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Fixative
    Formaldehyde

    Mr. Abderhman Abuhashem

    Verified customer

    Submitted Feb 18 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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