Key features and details
- Sheep polyclonal to NELFe
- Suitable for: ICC/IF, IP, WB
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Concentration information loading...
Our Abpromise guarantee covers the use of ab28038 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent dilution.|
|WB||1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).|
FunctionEssential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.
Tissue specificityWidely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the RRM NELF-E family.
Contains 1 RRM (RNA recognition motif) domain.
DomainThe RRM domain interacts with RNA, and is essential for NELF complex function. It is however not required for the NELF complex formation.
- Information by UniProt
- Major histocompatibility complex gene RD antibody
- Negative elongation factor E antibody
- Negative elongation factor polypeptide E antibody
All lanes : Anti-NELFe antibody (ab28038) at 1/1000 dilution
Lane 1 : NELFe-GST fusion protein
Lane 2 : HeLa cell nuclear extract
All lanes : donkey anti-sheep peroxidase linked at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
HeLa cells stained with: 1. HCM antibody ab28038 diluted 1/800 and donkey anti-sheep Alexa 647 secondary antibody diluted 1/1000 2. DAPI
ab28038 has not yet been referenced specifically in any publications.