Recombinant
Anti-NELFe antibody [EPR11600] (ab170104)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-NELFe antibody [EPR11600]
See all NELFe primary antibodies -
DescriptionRabbit monoclonal [EPR11600] to NELFe
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant fragment corresponding to Human NELFe.
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Positive control
- HeLa, T47-D, HepG2 or 293T lysate, HepG2 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR11600
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab170104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 44 kDa. | |
| ICC/IF | 1/100 - 1/250. | |
| IP | 1/10 - 1/100. |
Application notes
Is unsuitable for Flow Cyt or IHC-P.
Target
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FunctionEssential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.
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Tissue specificityWidely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.
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Sequence similaritiesBelongs to the RRM NELF-E family.
Contains 1 RRM (RNA recognition motif) domain. -
DomainThe RRM domain interacts with RNA, and is essential for NELF complex function. It is however not required for the NELF complex formation.
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Post-translational
modificationsSumoylated. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 7936 Human
- Entrez Gene: 27632 Mouse
- Entrez Gene: 294258 Rat
- Omim: 154040 Human
- SwissProt: P18615 Human
- SwissProt: P19426 Mouse
- Unigene: 423935 Human
- Unigene: 279907 Mouse
see all -
Alternative names
- Major histocompatibility complex gene RD antibody
- Negative elongation factor E antibody
- Negative elongation factor polypeptide E antibody
see all
Images
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![Western blot - Anti-NELFe antibody [EPR11600] (ab170104)](https://www.abcam.com/ps/datasheet/images/170/ab170104/NELFe-Primary-antibodies-ab170104-1.jpg)
Western blot - Anti-NELFe antibody [EPR11600] (ab170104)All lanes : Anti-NELFe antibody [EPR11600] (ab170104) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : T47-D cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit
at 1/2000 dilution
Predicted band size: 44 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-NELFe antibody [EPR11600] (ab170104)](https://www.abcam.com/ps/datasheet/images/170/ab170104/NELFe-Primary-antibodies-ab170104-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NELFe antibody [EPR11600] (ab170104)Immunofluorescence analysis of HepG2 cells labeling NELFe with ab170104 at a 1/100 dilution. -
![Immunoprecipitation - Anti-NELFe antibody [EPR11600] (ab170104)](https://www.abcam.com/ps/datasheet/images/170/ab170104/NELFe-Primary-antibodies-ab170104-4.jpg)
Immunoprecipitation - Anti-NELFe antibody [EPR11600] (ab170104)Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (lane 1) or 1X PBS (negative control) (lane 2) using ab170104, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Zhao Z et al. CDK9 and SPT5 proteins are specifically required for expression of herpes simplex virus 1 replication-dependent late genes. J Biol Chem 292:15489-15500 (2017). Read more (PubMed: 28743741) »
- Awwad SW et al. NELF-E is recruited to DNA double-strand break sites to promote transcriptional repression and repair. EMBO Rep 18:745-764 (2017). Read more (PubMed: 28336775) »
