Product nameAnti-NELFe antibody [EPR11600]
See all NELFe primary antibodies
DescriptionRabbit monoclonal [EPR11600] to NELFe
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human NELFe.
- HeLa, T47-D, HepG2 or 293T lysate, HepG2 cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab170104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 44 kDa.|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
FunctionEssential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.
Tissue specificityWidely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the RRM NELF-E family.
Contains 1 RRM (RNA recognition motif) domain.
DomainThe RRM domain interacts with RNA, and is essential for NELF complex function. It is however not required for the NELF complex formation.
- Information by UniProt
- Major histocompatibility complex gene RD antibody
- Negative elongation factor E antibody
- Negative elongation factor polypeptide E antibody
All lanes : Anti-NELFe antibody [EPR11600] (ab170104) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : T47-D cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit
at 1/2000 dilution
Predicted band size: 44 kDa
Immunofluorescence analysis of HepG2 cells labeling NELFe with ab170104 at a 1/100 dilution.
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (lane 1) or 1X PBS (negative control) (lane 2) using ab170104, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
This product has been referenced in:
- Zhao Z et al. CDK9 and SPT5 proteins are specifically required for expression of herpes simplex virus 1 replication-dependent late genes. J Biol Chem 292:15489-15500 (2017). Read more (PubMed: 28743741) »
- Awwad SW et al. NELF-E is recruited to DNA double-strand break sites to promote transcriptional repression and repair. EMBO Rep 18:745-764 (2017). Read more (PubMed: 28336775) »