Overview

  • Product name
    Anti-NELFe antibody [EPR11600]
    See all NELFe primary antibodies
  • Description
    Rabbit monoclonal [EPR11600] to NELFe
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human NELFe.

  • Positive control
    • HeLa, T47-D, HepG2 or 293T lysate, HepG2 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11600
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 44 kDa.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function
      Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.
    • Tissue specificity
      Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.
    • Sequence similarities
      Belongs to the RRM NELF-E family.
      Contains 1 RRM (RNA recognition motif) domain.
    • Domain
      The RRM domain interacts with RNA, and is essential for NELF complex function. It is however not required for the NELF complex formation.
    • Post-translational
      modifications
      Sumoylated.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • Major histocompatibility complex gene RD antibody
      • Negative elongation factor E antibody
      • Negative elongation factor polypeptide E antibody
      • NELF e antibody
      • NELF-E antibody
      • Nelfe antibody
      • NELFE_HUMAN antibody
      • Nuclear protein antibody
      • RD antibody
      • RD protein antibody
      • RD RNA binding protein antibody
      • RDP antibody
      • RNA-binding protein RD antibody
      see all

    Images

    • Western blot - Anti-NELFe antibody [EPR11600] (ab170104)
      Western blot - Anti-NELFe antibody [EPR11600] (ab170104)
      All lanes : Anti-NELFe antibody [EPR11600] (ab170104) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : T47-D cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit
      at 1/2000 dilution

      Predicted band size: 44 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-NELFe antibody [EPR11600] (ab170104)
      Immunocytochemistry/ Immunofluorescence - Anti-NELFe antibody [EPR11600] (ab170104)
      Immunofluorescence analysis of HepG2 cells labeling NELFe with ab170104 at a 1/100 dilution.
    • Immunoprecipitation - Anti-NELFe antibody [EPR11600] (ab170104)
      Immunoprecipitation - Anti-NELFe antibody [EPR11600] (ab170104)
      Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (lane 1) or 1X PBS (negative control) (lane 2) using ab170104, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    This product has been referenced in:
    • Zhao Z  et al. CDK9 and SPT5 proteins are specifically required for expression of herpes simplex virus 1 replication-dependent late genes. J Biol Chem 292:15489-15500 (2017). Read more (PubMed: 28743741) »

    See 1 Publication for this product

    Publishing research using ab170104? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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