Name: Anti-NELFe antibody [EPR11600]
Brand: RabMAb
SKU: ab170104

Product name

Anti-NELFe antibody [EPR11600]
See all NELFe primary antibodies
Description

Rabbit monoclonal [EPR11600] to NELFe
Host species

Rabbit
Tested applications

Suitable for: WB, ICC/IF, IP, Flow Cytmore details
Unsuitable for: IHC-P
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment corresponding to Human NELFe.
Positive control
- WB: HeLa, T47-D, HepG2 or 293T lysate, HepG2 cells. Mouse and Rat brain lysates. ICC/IF: HeLa cells. FC: HeLa cells. IP: HeLa cells.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR11600
Isotype

IgG
Research areas

Alternative Versions
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
Related Products
- Recombinant Human NELFe protein (ab104637)

Our Abpromise guarantee covers the use of ab170104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/5000. Predicted molecular weight: 44 kDa.
ICC/IF		1/500. For unpurified use at 1/100 - 1/250.
IP		1/10 - 1/100.
Flow Cyt		1/300.

Application notes

Is unsuitable for IHC-P.

Function

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.
Tissue specificity

Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.
Sequence similarities

Belongs to the RRM NELF-E family.
Contains 1 RRM (RNA recognition motif) domain.
Domain

The RRM domain interacts with RNA, and is essential for NELF complex function. It is however not required for the NELF complex formation.
Post-translational
modifications

Sumoylated.
Cellular localization

Nucleus.
Target information above from: UniProt accession P18615 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 7936 Human
- Entrez Gene: 27632 Mouse
- Entrez Gene: 294258 Rat
- Omim: 154040 Human
- SwissProt: P18615 Human
- SwissProt: P19426 Mouse
- Unigene: 423935 Human
- Unigene: 279907 Mouse
- Unigene: 653 Mouse
- Unigene: 16164 Rat
see all
Alternative names
- Major histocompatibility complex gene RD antibody
- Negative elongation factor E antibody
- Negative elongation factor polypeptide E antibody
- NELF e antibody
- NELF-E antibody
- Nelfe antibody
- NELFE_HUMAN antibody
- Nuclear protein antibody
- RD antibody
- RD protein antibody
- RD RNA binding protein antibody
- RDP antibody
- RNA-binding protein RD antibody
see all

Immunocytochemistry/ Immunofluorescence - Anti-NELFe antibody [EPR11600] (ab170104)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling NELFe with Purified ab170104 at 1:500 dilution (0.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunoprecipitation - Anti-NELFe antibody [EPR11600] (ab170104)

ab170104 (purified) at 1:20 dilution (2µg) immunoprecipitating NELFe in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab170104 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab170104 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-NELFe antibody [EPR11600] (ab170104)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling NELFe with Purified ab170104 at 1:300 dilution (1 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-NELFe antibody [EPR11600] (ab170104)

All lanes : Anti-NELFe antibody [EPR11600] (ab170104) at 1/5000 dilution (Purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat brain lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 44 kDa
Observed band size: 44 kDa
Western blot - Anti-NELFe antibody [EPR11600] (ab170104)

All lanes : Anti-NELFe antibody [EPR11600] (ab170104) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : T47-D cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit
at 1/2000 dilution

Predicted band size: 44 kDa

This image was generated using the unpurified version of the product.
Immunocytochemistry/ Immunofluorescence - Anti-NELFe antibody [EPR11600] (ab170104)

Immunofluorescence analysis of HepG2 cells labeling NELFe with ab170104 at a 1/100 dilution.

This image was generated using the unpurified version of the product.
Immunoprecipitation - Anti-NELFe antibody [EPR11600] (ab170104)

Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (lane 1) or 1X PBS (negative control) (lane 2) using ab170104, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

This image was generated using the unpurified version of the product.

Western blot protocols
Immunoprecipitation protocols
Immunocytochemistry & immunofluorescence protocols

Datasheet

This product has been referenced in:

Zhao Z et al. CDK9 and SPT5 proteins are specifically required for expression of herpes simplex virus 1 replication-dependent late genes. J Biol Chem 292:15489-15500 (2017). Read more (PubMed: 28743741) »
Awwad SW et al. NELF-E is recruited to DNA double-strand break sites to promote transcriptional repression and repair. EMBO Rep 18:745-764 (2017). Read more (PubMed: 28336775) »

See all 2 Publications for this product

Publishing research using ab170104? Please let us know so that we can cite the reference in this datasheet.

Location selector

Your location is currently set to:

Custom solutions & partnerships

Anti-NELFe antibody [EPR11600] (ab170104)

Overview

Properties

Associated products

Applications

Target

Images

Protocols

Datasheets and documents

References

This product has been referenced in:

Customer reviews and Q&As