Application
Western blot
Sample
Human Cell lysate - whole cell (hepatocellular carcinoma cells)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Gel)
Loading amount
2 µg
Specification
hepatocellular carcinoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1X PBST
Dilution
1/2500
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: HRP Conjugated Secondary anti-rabbit
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 49 kDa
Positive control
N/A
Abcam user community
Verified customer
Submitted Oct 02 2019