Recombinant
RabMAb

Recombinant Anti-NELFe antibody [EPR11600] - BSA and Azide free (ab240158)

Overview

  • Product name
    Anti-NELFe antibody [EPR11600] - BSA and Azide free
    See all NELFe primary antibodies
  • Description
    Rabbit monoclonal [EPR11600] to NELFe - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, ICC/IF, WB, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human NELFe.

  • Positive control
    • ICC/IF: HeLa cells. FC: HeLa cells. IP: HeLa cells.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    ab240158 is a PBS-only buffer format of ab170104. Please refer to ab170104 for recommended dilutions, protocols, and image data.

     

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Affinity purified
  • Clonality
    Monoclonal
  • Clone number
    EPR11600
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab240158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 44 kDa.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.
    • Tissue specificity
      Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.
    • Sequence similarities
      Belongs to the RRM NELF-E family.
      Contains 1 RRM (RNA recognition motif) domain.
    • Domain
      The RRM domain interacts with RNA, and is essential for NELF complex function. It is however not required for the NELF complex formation.
    • Post-translational
      modifications
      Sumoylated.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Major histocompatibility complex gene RD antibody
      • Negative elongation factor E antibody
      • Negative elongation factor polypeptide E antibody
      • NELF e antibody
      • NELF-E antibody
      • Nelfe antibody
      • NELFE_HUMAN antibody
      • Nuclear protein antibody
      • RD antibody
      • RD protein antibody
      • RD RNA binding protein antibody
      • RDP antibody
      • RNA-binding protein RD antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling NELFe with Purified ab170104 at 1:500 dilution (0.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170104).

       

    • ab170104 (purified) at 1:20 dilution (2µg) immunoprecipitating NELFe in HeLa whole cell lysate.
      Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab170104 & HeLa whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab170104 in HeLa whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170104).

       

    • Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling NELFe with Purified ab170104 at 1:300 dilution (1 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170104).

       

    • Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (lane 1) or 1X PBS (negative control) (lane 2) using ab170104, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170104).

      This image was generated using the unpurified version of the product. 

    • Immunofluorescence analysis of HepG2 cells labeling NELFe with ab170104 at a 1/100 dilution.

      This image was generated using the unpurified version of the product. 

    References

    ab240158 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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