Anti-NEMF antibody (ab241222)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-NEMF antibody
See all NEMF primary antibodies -
Description
Rabbit polyclonal to NEMF -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human NEMF aa 950-1000. The exact sequence is proprietary.
Database link: O60524 -
Positive control
- IP: Jurkat whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab241222 was affinity purified using an epitope specific to NEMF immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab241222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. Use 8 - 15 µl/mg lysate. |
Target
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Function
Plays a role in nuclear export. -
Tissue specificity
Expressed in brain, heart, liver, lung, spleen, and skeletal muscle. Also expressed at lower levels in stomach and testis. -
Sequence similarities
Belongs to the NEMF family. -
Domain
The N-terminal domain contains a nuclear export signal. The C-terminal domain may interact with cargo proteins. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9147 Human
- Omim: 608378 Human
- SwissProt: O60524 Human
- Unigene: 655964 Human
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Alternative names
- 1500011I12Rik antibody
- 4933405E14Rik antibody
- Antigen NY-CO-1 antibody
see all
Images
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Immunoprecipitation - Anti-NEMF antibody (ab241222)NEMF was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer, using ab241222 at 8 µl per reaction. Western blot was performed on the immunoprecipitate using ab241222 at 1/400 dilution.
Lane 1: ab241222 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Datasheets and documents
References
ab241222 has not yet been referenced specifically in any publications.
