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    nemf-antibody-ab241222.pdf

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Anti-NEMF antibody (ab241222)

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Immunoprecipitation - Anti-NEMF antibody (ab241222)

    Key features and details

    • Rabbit polyclonal to NEMF
    • Suitable for: IP
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-NEMF antibody
      See all NEMF primary antibodies
    • Description

      Rabbit polyclonal to NEMF
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IPmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide within Human NEMF aa 950-1000. The exact sequence is proprietary.
      Database link: O60524

    • Positive control

      • IP: Jurkat whole cell lysate.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: Tris buffered saline, 0.1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab241222 was affinity purified using an epitope specific to NEMF immobilized on solid support.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cancer
      • Tumor biomarkers
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Jurkat whole cell lysate (ab30128)
      • Jurkat whole cell lysate (ab7899)

    Applications

    Our Abpromise guarantee covers the use of ab241222 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IP Use at an assay dependent concentration.

    Use 8 - 15 µl/mg lysate.

    Target

    • Function

      Plays a role in nuclear export.
    • Tissue specificity

      Expressed in brain, heart, liver, lung, spleen, and skeletal muscle. Also expressed at lower levels in stomach and testis.
    • Sequence similarities

      Belongs to the NEMF family.
    • Domain

      The N-terminal domain contains a nuclear export signal. The C-terminal domain may interact with cargo proteins.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession O60524 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 9147 Human
      • Omim: 608378 Human
      • SwissProt: O60524 Human
      • Unigene: 655964 Human
      • Alternative names

        • 1500011I12Rik antibody
        • 4933405E14Rik antibody
        • Antigen NY-CO-1 antibody
        • FLJ10051 antibody
        • Nemf antibody
        • NEMF_HUMAN antibody
        • Nuclear export mediator factor Nemf antibody
        • NY CO 1 antibody
        • SDCCAG1 antibody
        • SDCG1 antibody
        • Serologically defined colon cancer antigen 1 antibody
        see all

      Images

      • Immunoprecipitation - Anti-NEMF antibody (ab241222)
        Immunoprecipitation - Anti-NEMF antibody (ab241222)

        NEMF was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer, using ab241222 at 8 µl per reaction. Western blot was performed on the immunoprecipitate using ab241222 at 1/400 dilution.

        Lane 1: ab241222 IP in Jurkat whole cell lysate.
        Lane 2: Control IgG IP in Jurkat whole cell lysate.


        Detection: Chemiluminescence with an exposure time of 10 seconds.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab241222? Please let us know so that we can cite the reference in this datasheet.

      ab241222 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab241222.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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