Anti-NEMF antibody (ab241222)
Key features and details
- Rabbit polyclonal to NEMF
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NEMF antibody
See all NEMF primary antibodies -
Description
Rabbit polyclonal to NEMF -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human NEMF aa 950-1000. The exact sequence is proprietary.
Database link: O60524 -
Positive control
- IP: Jurkat whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab241222 was affinity purified using an epitope specific to NEMF immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab241222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. Use 8 - 15 µl/mg lysate. |
Target
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Function
Plays a role in nuclear export. -
Tissue specificity
Expressed in brain, heart, liver, lung, spleen, and skeletal muscle. Also expressed at lower levels in stomach and testis. -
Sequence similarities
Belongs to the NEMF family. -
Domain
The N-terminal domain contains a nuclear export signal. The C-terminal domain may interact with cargo proteins. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9147 Human
- Omim: 608378 Human
- SwissProt: O60524 Human
- Unigene: 655964 Human
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Alternative names
- 1500011I12Rik antibody
- 4933405E14Rik antibody
- Antigen NY-CO-1 antibody
see all
Images
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Immunoprecipitation - Anti-NEMF antibody (ab241222)
NEMF was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer, using ab241222 at 8 µl per reaction. Western blot was performed on the immunoprecipitate using ab241222 at 1/400 dilution.
Lane 1: ab241222 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab241222 has not yet been referenced specifically in any publications.
