Key features and details
- Rabbit polyclonal to NENF
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-NENF antibody
DescriptionRabbit polyclonal to NENF
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (Human) of 15 amino acids from near the C-terminus of NENF. (NP_037481).
- Human kidney tissue lysate
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 19 kDa).|
|IHC-P||Use a concentration of 10 µg/ml.|
|ICC/IF||Use a concentration of 20 µg/ml.|
FunctionDisplays neurotrophic activity and activates phosphorylation of MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT in primary cultured neurons. Does not have mitogenic activity in primary cultured astrocytes. May play a role on neuronal differentiation and may have a transient effect on neural cell proliferation in neural precursor cells. Neurotrophic activity is enhanced by binding to heme.
Sequence similaritiesBelongs to the cytochrome b5 family. MAPR subfamily.
Contains 1 cytochrome b5 heme-binding domain.
DomainThe cytochrome b5 heme-binding domain was proven to bind heme, although it lacks the conserved iron-binding His residue at position 82.
Cellular localizationSecreted > extracellular space.
- Information by UniProt
- Cell growth inhibiting protein 47 antibody
- Cell immortalization-related protein 2 antibody
- CIR2 antibody
Lane 1 : Anti-NENF antibody (ab74474) at 1 µg/ml
Lane 2 : Anti-NENF antibody (ab74474) at 2 µg/ml
All lanes : Human kidney tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 19 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Immunofluorescence of NENF in rat kidney tissue with ab74474 at 20 ug/mL.
ab74474 at 10 µg/ml staining NENF in rat kidney tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
ab74474 has been referenced in 1 publication.
- Stefanska B et al. Genome-wide study of hypomethylated and induced genes in patients with liver cancer unravels novel anticancer targets. Clin Cancer Res 20:3118-32 (2014). WB ; Human . PubMed: 24763612