Overview

  • Product name

    Anti-NENF antibody
  • Description

    Rabbit polyclonal to NENF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human) of 15 amino acids from near the C-terminus of NENF. (NP_037481).

  • Positive control

    • Human kidney tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab74474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 19 kDa).
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.

Target

  • Function

    Displays neurotrophic activity and activates phosphorylation of MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT in primary cultured neurons. Does not have mitogenic activity in primary cultured astrocytes. May play a role on neuronal differentiation and may have a transient effect on neural cell proliferation in neural precursor cells. Neurotrophic activity is enhanced by binding to heme.
  • Sequence similarities

    Belongs to the cytochrome b5 family. MAPR subfamily.
    Contains 1 cytochrome b5 heme-binding domain.
  • Domain

    The cytochrome b5 heme-binding domain was proven to bind heme, although it lacks the conserved iron-binding His residue at position 82.
  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cell growth inhibiting protein 47 antibody
    • Cell immortalization-related protein 2 antibody
    • CIR2 antibody
    • Nenf antibody
    • NENF_HUMAN antibody
    • Neudesin antibody
    • Neuron derived neurotrophic factor antibody
    • Neuron-derived neurotrophic factor antibody
    • SCIRP10 antibody
    • SCIRP10 related protein antibody
    • Secreted protein of unknown function antibody
    • Spinal cord injury related protein 10 antibody
    • SPUF antibody
    • SPUF protein antibody
    see all

Images

  • Lane 1 : Anti-NENF antibody (ab74474) at 1 µg/ml
    Lane 2 : Anti-NENF antibody (ab74474) at 2 µg/ml

    All lanes : Human kidney tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 19 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?

  • Immunofluorescence of NENF in rat kidney tissue with ab74474 at 20 ug/mL.

  • ab74474 at 10 µg/ml staining NENF in rat kidney tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

References

This product has been referenced in:

  • Stefanska B  et al. Genome-wide study of hypomethylated and induced genes in patients with liver cancer unravels novel anticancer targets. Clin Cancer Res 20:3118-32 (2014). WB ; Human . Read more (PubMed: 24763612) »
See 1 Publication for this product

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