Key features and details
- Rabbit polyclonal to Nephrin
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-Nephrin antibody
See all Nephrin primary antibodies
DescriptionRabbit polyclonal to Nephrin
Tested applicationsSuitable for: Flow Cyt, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
- mouse kidney tissue; Rat RSC96 cells
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab216692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.|
FunctionSeems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.
Tissue specificitySpecifically expressed in podocytes of kidney glomeruli.
Involvement in diseaseDefects in NPHS1 are the cause of nephrotic syndrome type 1 (NPHS1) [MIM:256300]; also known as Finnish congenital nephrosis (CNF). A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure.
Sequence similaritiesBelongs to the immunoglobulin superfamily.
Contains 1 fibronectin type-III domain.
Contains 8 Ig-like C2-type (immunoglobulin-like) domains.
Developmental stageIn 23-week-old embryo found in epithelial podocytes of the periphery of mature and developing glomeruli.
modificationsPhosphorylated on tyrosine residues.
Cellular localizationCell membrane. Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane.
- Information by UniProt
- CNF antibody
- Nephrin antibody
- Nephrosis 1 congenital Finnish type antibody
Anti-Nephrin antibody (ab216692) at 1/500 dilution + MDCK cell lysates
Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse kidney tissue labeling Nephrin using ab216692 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Flow cytometric analysis of rat RSC96 cells labeling Nephrin using ab216692 at 1/100 dilution for 30 minutes (green), followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue) and isotype control (orange).
ab216692 has been referenced in 1 publication.
- Kuzu M et al. Protective effect of morin on doxorubicin-induced hepatorenal toxicity in rats. Chem Biol Interact 308:89-100 (2019). PubMed: 31100273