Anti-Nephrin antibody (ab216692)
Key features and details
- Rabbit polyclonal to Nephrin
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Mouse, Rat, Dog
- Isotype: IgG
Overview
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Product name
Anti-Nephrin antibody
See all Nephrin primary antibodies -
Description
Rabbit polyclonal to Nephrin -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Dog
Predicted to work with: Human -
Immunogen
Synthetic peptide within Human Nephrin aa 450-550 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O60500 -
Positive control
- mouse kidney tissue; Rat RSC96 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
1/100.
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|
IHC-P |
1/100 - 1/500.
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|
WB |
1/500.
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Notes |
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Flow Cyt
1/100. |
IHC-P
1/100 - 1/500. |
WB
1/500. |
Target
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Function
Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. -
Tissue specificity
Specifically expressed in podocytes of kidney glomeruli. -
Involvement in disease
Defects in NPHS1 are the cause of nephrotic syndrome type 1 (NPHS1) [MIM:256300]; also known as Finnish congenital nephrosis (CNF). A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure. -
Sequence similarities
Belongs to the immunoglobulin superfamily.
Contains 1 fibronectin type-III domain.
Contains 8 Ig-like C2-type (immunoglobulin-like) domains. -
Developmental stage
In 23-week-old embryo found in epithelial podocytes of the periphery of mature and developing glomeruli. -
Post-translational
modificationsPhosphorylated on tyrosine residues. -
Cellular localization
Cell membrane. Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 4868 Human
- Entrez Gene: 54631 Mouse
- Entrez Gene: 64563 Rat
- Omim: 602716 Human
- SwissProt: O60500 Human
- SwissProt: Q9QZS7 Mouse
- SwissProt: Q9R044 Rat
- Unigene: 122186 Human
see all -
Alternative names
- CNF antibody
- Nephrin antibody
- Nephrosis 1 congenital Finnish type antibody
see all
Images
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Anti-Nephrin antibody (ab216692) at 1/500 dilution + MDCK cell lysates
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Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse kidney tissue labeling Nephrin using ab216692 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Flow cytometric analysis of rat RSC96 cells labeling Nephrin using ab216692 at 1/100 dilution for 30 minutes (green), followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue) and isotype control (orange).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab216692 has been referenced in 4 publications.
- Jin D et al. Impact of klotho on the expression of SRGAP2a in podocytes in diabetic nephropathy. BMC Nephrol 23:151 (2022). PubMed: 35436879
- Song S et al. Sestrin2 remedies podocyte injury via orchestrating TSP-1/TGF-β1/Smad3 axis in diabetic kidney disease. Cell Death Dis 13:663 (2022). PubMed: 35908070
- Kuzu M et al. Protective effect of morin on doxorubicin-induced hepatorenal toxicity in rats. Chem Biol Interact 308:89-100 (2019). PubMed: 31100273
- Balkawade RS et al. Podocyte-specific expression of Cre recombinase promotes glomerular basement membrane thickening. Am J Physiol Renal Physiol 316:F1026-F1040 (2019). PubMed: 30810063