Key features and details
- Rabbit polyclonal to Nephrin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Nephrin antibody
See all Nephrin primary antibodies
DescriptionRabbit polyclonal to Nephrin
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Nephrin aa 23-257.
QLAIPASVPRGFWALPENLTVVEGASVELRCGVSTPGSAVQWAKDGLLLG PDPRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQVGRSEMGPEL VSPRVILSILVPPKLLLLTPEAGTMVTWVAGQEYVVNCVSGDAKPAPDIT ILLSGQTISDISANVNEGSQQKLFTVEATARVTPRSSDNRQLLVCEASSP ALEAPIKASFTVNVLFPPGPPVIEWPGLDEGHVRA
Database link: O60500
- IHC-P: Human kidney tissue. WB: Jurkat whole cell lysate. ICC/IF: HepG2 cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab235903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 134 kDa.|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionSeems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.
Tissue specificitySpecifically expressed in podocytes of kidney glomeruli.
Involvement in diseaseDefects in NPHS1 are the cause of nephrotic syndrome type 1 (NPHS1) [MIM:256300]; also known as Finnish congenital nephrosis (CNF). A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure.
Sequence similaritiesBelongs to the immunoglobulin superfamily.
Contains 1 fibronectin type-III domain.
Contains 8 Ig-like C2-type (immunoglobulin-like) domains.
Developmental stageIn 23-week-old embryo found in epithelial podocytes of the periphery of mature and developing glomeruli.
modificationsPhosphorylated on tyrosine residues.
Cellular localizationCell membrane. Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane.
- Information by UniProt
- CNF antibody
- Nephrin antibody
- Nephrosis 1 congenital Finnish type antibody
Anti-Nephrin antibody (ab235903) at 1/500 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 134 kDa
Paraffin-embedded human kidney tissue stained for Nephrin using ab235903 at 1/100 dilution in immunohistochemical analysis.
4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Nephrin (green) using ab235903 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235903 has not yet been referenced specifically in any publications.