Product nameAnti-Nephrin antibody - C-terminal
See all Nephrin primary antibodies
DescriptionRabbit polyclonal to Nephrin - C-terminal
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- Mouse spleen and mouse kidney tissue lysates; Human liver cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 49% PBS
(without Mg2+ and Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab183099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/25 - 1/100.|
|WB||1/500 - 1/2000. Predicted molecular weight: 134 kDa.|
FunctionSeems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.
Tissue specificitySpecifically expressed in podocytes of kidney glomeruli.
Involvement in diseaseDefects in NPHS1 are the cause of nephrotic syndrome type 1 (NPHS1) [MIM:256300]; also known as Finnish congenital nephrosis (CNF). A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure.
Sequence similaritiesBelongs to the immunoglobulin superfamily.
Contains 1 fibronectin type-III domain.
Contains 8 Ig-like C2-type (immunoglobulin-like) domains.
Developmental stageIn 23-week-old embryo found in epithelial podocytes of the periphery of mature and developing glomeruli.
modificationsPhosphorylated on tyrosine residues.
Cellular localizationCell membrane. Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane.
- Information by UniProt
- CNF antibody
- Nephrin antibody
- Nephrosis 1 congenital Finnish type antibody
All lanes : Anti-Nephrin antibody - C-terminal (ab183099) at 1/800 dilution
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lysates/proteins at 40 µg per lane.
All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/5000 dilution
Predicted band size: 134 kDa
Exposure time: 1 minute
Immunohistochemical analysis of paraffin embedded Human liver cancer tissue labeling Nephrin with ab183099 at 1/50 dilution. Image on the right is treated with the synthetic peptide.
This product has been referenced in:
- Rauch C et al. Differentiation of human iPSCs into functional podocytes. PLoS One 13:e0203869 (2018). Read more (PubMed: 30222766) »
- Wei MG et al. JiaWeiDangGui Decoction Ameliorates Proteinuria and Kidney Injury in Adriamycin-Induced Rat by Blockade of TGF-ß/Smad Signaling. Evid Based Complement Alternat Med 2016:5031890 (2016). IHC-P ; Rat . Read more (PubMed: 27403197) »