Overview

  • Product name

    Anti-Nephrin antibody - C-terminal
    See all Nephrin primary antibodies
  • Description

    Rabbit polyclonal to Nephrin - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human Nephrin aa 1229-1241 (C terminal). The exact sequence is proprietary. (NP_004637)
    Sequence:

    PDSLPFELRGHLV


    Database link: O60500

  • Positive control

    • Mouse spleen and mouse kidney tissue lysates; Human liver cancer tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 49% PBS

    (without Mg2+ and Ca2+)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/100.
WB 1/500 - 1/2000. Predicted molecular weight: 134 kDa.

Target

  • Function

    Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.
  • Tissue specificity

    Specifically expressed in podocytes of kidney glomeruli.
  • Involvement in disease

    Defects in NPHS1 are the cause of nephrotic syndrome type 1 (NPHS1) [MIM:256300]; also known as Finnish congenital nephrosis (CNF). A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily.
    Contains 1 fibronectin type-III domain.
    Contains 8 Ig-like C2-type (immunoglobulin-like) domains.
  • Developmental stage

    In 23-week-old embryo found in epithelial podocytes of the periphery of mature and developing glomeruli.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues.
  • Cellular localization

    Cell membrane. Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CNF antibody
    • Nephrin antibody
    • Nephrosis 1 congenital Finnish type antibody
    • Nephrosis 1, congenital, Finnish type (nephrin) antibody
    • NPHN antibody
    • NPHN_HUMAN antibody
    • NPHS 1 antibody
    • Nphs1 antibody
    • Renal glomerulus specific cell adhesion receptor antibody
    • Renal glomerulus-specific cell adhesion receptor antibody
    see all

Images

  • All lanes : Anti-Nephrin antibody - C-terminal (ab183099) at 1/800 dilution

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : Mouse kidney tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/5000 dilution

    Predicted band size: 134 kDa


    Exposure time: 1 minute


    8% SDS-PAGE

  • Immunohistochemical analysis of paraffin embedded Human liver cancer tissue labeling Nephrin with ab183099 at 1/50 dilution. Image on the right is treated with the synthetic peptide.

References

This product has been referenced in:

  • Rauch C  et al. Differentiation of human iPSCs into functional podocytes. PLoS One 13:e0203869 (2018). Read more (PubMed: 30222766) »
  • Wei MG  et al. JiaWeiDangGui Decoction Ameliorates Proteinuria and Kidney Injury in Adriamycin-Induced Rat by Blockade of TGF-ß/Smad Signaling. Evid Based Complement Alternat Med 2016:5031890 (2016). IHC-P ; Rat . Read more (PubMed: 27403197) »
See all 3 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Western blot
Sample
Mouse Tissue lysate - whole (Kidney)
Gel Running Conditions
Reduced Denaturing (4-12)
Loading amount
65 µg
Specification
Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted Apr 10 2017

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