Overview

  • Product name

    Anti-Nephrin antibody [Y17-R]
    See all Nephrin primary antibodies
  • Description

    Rabbit monoclonal [Y17-R] to Nephrin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from the centre of the fifth Ig-like domain of Human Nephrin (between residues Cys465-Cys528).

  • Epitope

    Antibody recognizes the epitope located between Cys465 - Cys528

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 8.00
    Preservative: 0.05% Sodium azide
    Constituents: 0.32% Tris HCl, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. This antibody is purified using a proprietary technique and offers a completely post-translationally modified and properly glycosylated antibody. This offers increased stability.
  • Clonality

    Monoclonal
  • Clone number

    Y17-R
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab136894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/5000. Predicted molecular weight: 134 kDa.
IP Use at an assay dependent concentration.

Target

  • Function

    Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.
  • Tissue specificity

    Specifically expressed in podocytes of kidney glomeruli.
  • Involvement in disease

    Defects in NPHS1 are the cause of nephrotic syndrome type 1 (NPHS1) [MIM:256300]; also known as Finnish congenital nephrosis (CNF). A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily.
    Contains 1 fibronectin type-III domain.
    Contains 8 Ig-like C2-type (immunoglobulin-like) domains.
  • Developmental stage

    In 23-week-old embryo found in epithelial podocytes of the periphery of mature and developing glomeruli.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues.
  • Cellular localization

    Cell membrane. Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CNF antibody
    • Nephrin antibody
    • Nephrosis 1 congenital Finnish type antibody
    • Nephrosis 1, congenital, Finnish type (nephrin) antibody
    • NPHN antibody
    • NPHN_HUMAN antibody
    • NPHS 1 antibody
    • Nphs1 antibody
    • Renal glomerulus specific cell adhesion receptor antibody
    • Renal glomerulus-specific cell adhesion receptor antibody
    see all

Images

  • All lanes : Anti-Nephrin antibody [Y17-R] (ab136894) at 1/5000 dilution

    Lane 1 : Recombinant Mouse Nephrin (extracellular domain, Gln 37 – Thr 1049) - 50ng
    Lane 2 : Recombinant Mouse Nephrin (extracellular domain, Gln 37 – Thr 1049) - 100ng
    Lane 3 : Recombinant Mouse Nephrin (extracellular domain, Gln 37 – Thr 1049) - 200ng

    Predicted band size: 134 kDa

  • Overlay histogram showing HEK293 cells stained with ab136894 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab136894, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 80% methanol (5 min) fixed HEK293 cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References

This product has been referenced in:

  • Yang H  et al. Tim-3 aggravates podocyte injury in diabetic nephropathy by promoting macrophage activation via the NF-?B/TNF-a pathway. Mol Metab 23:24-36 (2019). Read more (PubMed: 30862474) »
  • Shi W  et al. Protective effect of calcitriol on podocytes in spontaneously hypertensive rat. J Chin Med Assoc 81:691-698 (2018). Read more (PubMed: 29748075) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (kidney, podocyte)
Permeabilization
No
Specification
kidney, podocyte
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 23°C
Fixative
Paraformaldehyde

Miss. Kara Shumansky

Verified customer

Submitted May 06 2019

Application
Western blot
Sample
Mouse Tissue lysate - whole (Kidney)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
60 µg
Specification
Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Wesley Konsavage

Verified customer

Submitted Jan 04 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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