Key features and details
- Rabbit monoclonal [EPTPG1] to Nephrin (phospho Y1176 + Y1193)
- Suitable for: WB, Dot blot
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1]
See all Nephrin primary antibodies
DescriptionRabbit monoclonal [EPTPG1] to Nephrin (phospho Y1176 + Y1193)
SpecificityThis antibody detects Nephrin when either tyrosines 1176 or 1193 are phosphorylated, but does not detect non-phosphorylated Nephrin.
Tested applicationsSuitable for: WB, Dot blotmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Mouse Nephrin (phospho Y1176 + Y1193). The exact sequence is proprietary.
- Transfected HEK293T cells
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab80299 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 138, 180 kDa (predicted molecular weight: 138 kDa).|
|Dot blot||Use at an assay dependent concentration.|
FunctionSeems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.
Tissue specificitySpecifically expressed in podocytes of kidney glomeruli.
Involvement in diseaseDefects in NPHS1 are the cause of nephrotic syndrome type 1 (NPHS1) [MIM:256300]; also known as Finnish congenital nephrosis (CNF). A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure.
Sequence similaritiesBelongs to the immunoglobulin superfamily.
Contains 1 fibronectin type-III domain.
Contains 8 Ig-like C2-type (immunoglobulin-like) domains.
Developmental stageIn 23-week-old embryo found in epithelial podocytes of the periphery of mature and developing glomeruli.
modificationsPhosphorylated on tyrosine residues.
Cellular localizationCell membrane. Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane.
- Information by UniProt
- CNF antibody
- Nephrin antibody
- Nephrosis 1 congenital Finnish type antibody
All lanes : Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299) at 1/1000 dilution
Lane 1 : HEK293T (human embryonic kidney epithelial cell) transfected with myc-tagged human Nephrin expression vector, whole cell lysate
Lane 2 : HEK293T (human embryonic kidney epithelial cell) transfected with myc-tagged human Nephrin and myc-tagged Src expression vector, whole cell lysate
Lane 3 : HEK293T (human embryonic kidney epithelial cell) transfected with myc-tagged human Nephrin and myc-tagged Src expression vector, whole cell lysate. Then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 138 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Blocking and dilution buffer: 5% NFDM/TBST.
Dot blot analysis of Nephrin (pY1176 + pY1193) phospho peptide (Lane 1), Nephrin (pY1176) phospho peptide (Lane 2), Nephrin (pY1193) phospho peptide (Lane 3) and Nephrin non-phospho peptide (Lane 4) labelling Nephrin (pY1176 + pY1193) with ab80299 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.
All lanes : Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299) at 1/50000 dilution
Lane 1 : HEK293T (human embryonic kidney epithelial cell) transfected with wild type CD 16/7 Nephrin expression vector, whole cell lysate
Lane 2 : HEK293T (human embryonic kidney epithelial cell) transfected with wild type CD 16/7 Nephrin and active tyrosine kinase (v-Src) expression vector, whole cell lysate
Lane 3 : HEK293T (human embryonic kidney epithelial cell) transfected with Y1176F mutant CD 16/7 Nephrin and active tyrosine kinase (v-Src) expression vector, whole cell lysate
Lane 4 : HEK293T (human embryonic kidney epithelial cell) transfected with Y1193F mutant CD 16/7 Nephrin and active tyrosine kinase (v-Src) expression vector, whole cell lysate
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 138 kDa
Observed band size: 138,180 kDa why is the actual band size different from the predicted?
ab80299 has been referenced in 5 publications.
- Chun J et al. Recruitment of APOL1 kidney disease risk variants to lipid droplets attenuates cell toxicity. Proc Natl Acad Sci U S A 116:3712-3721 (2019). PubMed: 30733285
- Wei C et al. SHROOM3-FYN Interaction Regulates Nephrin Phosphorylation and Affects Albuminuria in Allografts. J Am Soc Nephrol 29:2641-2657 (2018). PubMed: 30341149
- Lin JS et al. ARF6 mediates nephrin tyrosine phosphorylation-induced podocyte cellular dynamics. PLoS One 12:e0184575 (2017). PubMed: 28880939
- Kapodistria K et al. Nephrin, a transmembrane protein, is involved in pancreatic beta-cell survival signaling. Mol Cell Endocrinol 400:112-28 (2015). PubMed: 25448064
- Jeon J et al. Dynamin-mediated Nephrin Phosphorylation Regulates Glucose-stimulated Insulin Release in Pancreatic Beta Cells. J Biol Chem 287:28932-42 (2012). WB ; Human . PubMed: 22718751