Recombinant Anti-Nephrin (phospho Y1217) antibody [EPTPG3] - BSA and Azide free (ab239894)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPTPG3] to Nephrin (phospho Y1217) - BSA and Azide free
- Suitable for: Dot blot, WB
- Reacts with: Human
Overview
-
Product name
Anti-Nephrin (phospho Y1217) antibody [EPTPG3] - BSA and Azide free
See all Nephrin primary antibodies -
Description
Rabbit monoclonal [EPTPG3] to Nephrin (phospho Y1217) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: 293T transfected with myc-tagged human Nephrin and myc-tagged Src expression vectors.
-
General notes
ab239894 is the carrier-free version of ab80298.
This product has switched from a hybridoma to recombinant production method on 20th November 2023.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPTPG3 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab239894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Dot blot |
Use at an assay dependent concentration.
|
|
WB |
Use at an assay dependent concentration. Detects a band of approximately 138 kDa (predicted molecular weight: 138 kDa).
|
Notes |
---|
Dot blot
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 138 kDa (predicted molecular weight: 138 kDa). |
Target
-
Function
Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. -
Tissue specificity
Specifically expressed in podocytes of kidney glomeruli. -
Involvement in disease
Defects in NPHS1 are the cause of nephrotic syndrome type 1 (NPHS1) [MIM:256300]; also known as Finnish congenital nephrosis (CNF). A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure. -
Sequence similarities
Belongs to the immunoglobulin superfamily.
Contains 1 fibronectin type-III domain.
Contains 8 Ig-like C2-type (immunoglobulin-like) domains. -
Developmental stage
In 23-week-old embryo found in epithelial podocytes of the periphery of mature and developing glomeruli. -
Post-translational
modificationsPhosphorylated on tyrosine residues. -
Cellular localization
Cell membrane. Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane. - Information by UniProt
-
Database links
- Entrez Gene: 4868 Human
- Entrez Gene: 54631 Mouse
- Omim: 602716 Human
- SwissProt: O60500 Human
- SwissProt: Q9QZS7 Mouse
- Unigene: 122186 Human
- Unigene: 437830 Mouse
-
Alternative names
- CNF antibody
- Nephrin antibody
- Nephrosis 1 congenital Finnish type antibody
see all
Images
-
All lanes : Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (ab80298) at 1/10000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) transfected with a myc-tagged empty vector whole cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) transfected with a myc-tagged human Nephrin expression vector, whole cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) transfected with a myc-tagged Src expression vector, whole cell lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) transfected with myc-tagged human Nephrin and myc-tagged Src expression vectors, whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 138 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
-
All lanes : Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (ab80298) at 1/10000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) transfected with a myc-tagged empty vector whole cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) transfected with with myc-tagged human Nephrin and myc-tagged Src expression vectors, whole cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) transfected with myc-tagged human Nephrin and myc-tagged Src expression vectors, whole cell lysate 15 µg. Then the membrane was incubated with Alkaline phosphatase
Lysates/proteins at 15 µg per lane.
Predicted band size: 138 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
-
Dot blot analysis of Nephrin (pY1217) peptide and Nephrin non-phospho peptide labelling PKC alpha Nephrin (pY1217) with ab80298 at a 1/1000 dilution. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a 1/20,000 dilution. Blocking and dilution buffer: 5% NFDM/TBST.
Lane 1: Nephrin (pY1217) phospho peptide
Lane 2: Nephrin non-phospho peptide
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab239894 has not yet been referenced specifically in any publications.