Key features and details
- Rabbit polyclonal to Neprilysin-2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Neprilysin-2 antibody
See all Neprilysin-2 primary antibodies
DescriptionRabbit polyclonal to Neprilysin-2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog
- WB: MCF7 cell lysate and fetal liver lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified by peptide affinity chromatography method.
Our Abpromise guarantee covers the use of ab81688 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 89 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionMetalloprotease involved in sperm function, possibly by modulating the processes of fertilization and early embryonic development. Degrades a broad variety of small peptides with a preference for peptides shorter than 3 kDa containing neutral bulky aliphatic or aromatic amino acid residues. Shares the same substrate specificity with MME and cleaves peptides at the same amide bond.
Tissue specificityPredominantly expressed in testis. Weakly expressed in brain, kidney and heart.
Sequence similaritiesBelongs to the peptidase M13 family.
Cellular localizationMembrane. Secreted. A secreted form produced by proteollytic cleavage also exists.
- Information by UniProt
- Mel transforming oncogene like 1 antibody
- Membrane metallo endopeptidase like 1 antibody
- Membrane metallo endopeptidase like 2 antibody
Anti-Neprilysin-2 antibody (ab81688) at 1 µg/ml + Fetal liver lysate at 10 µg with in 5% skim milk / PBS buffer
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 89 kDa
Observed band size: 89 kDa
Western blot analysis of fetal liver lysate labeling Neprilysin-2 with ab81688.
All lanes : Anti-Neprilysin-2 antibody (ab81688) at 1 µg/ml
Lane 1 : MCF7 cell lysate
Lane 2 : NTERA-2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 89 kDa
Western blot analysis labeling Neprilysin-2 with ab81688.
ab81688 has been referenced in 1 publication.
- Fu W et al. Tongluo Xingnao effervescent tablet reverses memory deficit and reduces plaque load in APPswe/PS1dE9 mice. Exp Ther Med 15:4005-4013 (2018). PubMed: 29563991