• Product name
  • Description
    Rabbit polyclonal to Nesprin 2
  • Host species
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 6350 - 6450 of Human Nesprin 2.

  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: human normal kidney.



Our Abpromise guarantee covers the use of ab103020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Connects nuclei to the cytoskeleton by interacting with the nuclear envelope and with F-actin in the cytoplasm. Specifically, SYNE2 and SUN2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. Required for centrosome migration to the apical cell surface during early ciliogenesis.
  • Tissue specificity
    Widely expressed, with higher level in kidney, adult and fetal liver, stomach and placenta. Weakly expressed in skeletal muscle and brain. Isoform 5 is highly expressed in pancreas, skeletal muscle and heart.
  • Involvement in disease
    Defects in SYNE2 are the cause of Emery-Dreifuss muscular dystrophy type 5 (EDMD5) [MIM:612999]. A degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows, Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects.
  • Sequence similarities
    Belongs to the nesprin family.
    Contains 1 actin-binding domain.
    Contains 2 CH (calponin-homology) domains.
    Contains 1 KASH domain.
    Contains 9 spectrin repeats.
  • Domain
    The KASH domain mediates the nuclear envelope targeting.
  • Cellular localization
    Nucleus outer membrane. Sarcoplasmic reticulum membrane. Cell membrane. Cytoplasm > cytoskeleton. Mitochondrion. Nucleus > nucleoplasm. Different isoform patterns are found in the different compartments of the cell. The isoforms having the C-terminal transmembrane span can be found in several organellar membranes like the nuclear envelope, the sarcoplasmic reticulum of myoblasts, or the lamellipodia and focal adhesions at the cell membrane. The largest part of the outer nuclear membrane-associated protein is cytoplasmic, while its C-terminal part is associated with the nuclear envelope, most probably the outer nuclear membrane. Remains associated with the nuclear envelope during its breakdown in mitotic cells. Shorter solubles isoforms can be found in the cytoplasm and within the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZP434H2235 antibody
    • DKFZp686E01115 antibody
    • DKFZp686H1931 antibody
    • FLJ11014 antibody
    • FLJ43727 antibody
    • FLJ45710 antibody
    • FLJ46790 antibody
    • KIAA1011 antibody
    • Nesprin-2 antibody
    • Nesprin2 antibody
    • NUA antibody
    • NUANCE antibody
    • Nuclear envelope spectrin repeat protein 2 antibody
    • Nucleus and actin connecting element antibody
    • Nucleus and actin connecting element protein antibody
    • Protein NUANCE antibody
    • Spectrin repeat containing nuclear envelope 2 antibody
    • Synaptic nuclear envelope protein 2 antibody
    • Synaptic nuclei expressed gene 2 antibody
    • SYNE 2 antibody
    • Syne-2 antibody
    • SYNE2 antibody
    • SYNE2_HUMAN antibody
    see all


  • IHC image of Nesprin 2 staining in human normal kidney FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab103020, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


ab103020 has not yet been referenced specifically in any publications.

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