Recombinant
RabMAb

Recombinant Anti-Nesprin1/Syne-1 antibody [EPR14196] - BSA and Azide free (ab240327)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14196] to Nesprin1/Syne-1 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Nesprin1/Syne-1 antibody [EPR14196] - BSA and Azide free
    See all Nesprin1/Syne-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14196] to Nesprin1/Syne-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Nesprin1/Syne-1 aa 8250-8350. The exact sequence is proprietary.
    Database link: Q8NF91

  • General notes

    Ab240327 is the carrier-free version of ab192234. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240327 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14196

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab240327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Connects nuclei to the cytoskeleton by interacting with the nuclear envelope and with F-actin in the cytoplasm. Required for centrosome migration to the apical cell surface during early ciliogenesis.

  • Tissue specificity

    Widely expressed. Highly expressed in skeletal and smooth muscles, heart, spleen, and peripheral blood leukocytes.

  • Involvement in disease

    Defects in SYNE1 are the cause of spinocerebellar ataxia autosomal recessive type 8 (SCAR8) [MIM:610743]; also known as autosomal recessive cerebellar ataxia type 1 (ARCA1) or recessive ataxia of Beauce. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCAR8 is an autosomal recessive form.
    Defects in SYNE1 are the cause of Emery-Dreifuss muscular dystrophy type 4 (EDMD4) [MIM:612998]. A degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows, Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects.

  • Sequence similarities

    Belongs to the nesprin family.
    Contains 1 actin-binding domain.
    Contains 2 CH (calponin-homology) domains.
    Contains 12 HAT repeats.
    Contains 1 KASH domain.
    Contains 31 spectrin repeats.

  • Domain

    The KASH domain, which contains a transmembrane domain, mediates the nuclear envelope targeting and is involved in the binding to SUN1 and SUN2 through recognition of their SUN domains.

  • Cellular localization

    Nucleus outer membrane. Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere. The largest part of the protein is cytoplasmic, while its C-terminal part is associated with the nuclear envelope, most probably the outer nuclear membrane. In skeletal and smooth muscles, a significant amount is found in the sarcomeres.
  • Information by UniProt

  • Database links

    • Alternative names

      • 8B antibody
      • ARCA1 antibody
      • C6orf98 antibody
      • CPG2 antibody
      • CPG2 full length antibody
      • dJ45H2.2 antibody
      • EDMD4 antibody
      • Enaptin antibody
      • Myne-1 antibody
      • MYNE1 antibody
      • Myocyte nuclear envelope protein 1 antibody
      • Nesp1 antibody
      • Nesprin 1 antibody
      • Nesprin-1 antibody
      • Nuclear envelope spectrin repeat protein 1 antibody
      • SCAR8 antibody
      • Spectrin repeat containing nuclear envelope 1 antibody
      • Synaptic nuclear envelope protein 1 antibody
      • Synaptic nuclei expressed gene 1 antibody
      • Syne-1 antibody
      • SYNE1 antibody
      • SYNE1_HUMAN antibody
      • SYNE1B antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Nesprin1/Syne-1 antibody [EPR14196] - BSA and Azide free (ab240327)
      Immunocytochemistry/ Immunofluorescence - Anti-Nesprin1/Syne-1 antibody [EPR14196] - BSA and Azide free (ab240327)

      Immunofluorescent analysis of A673 cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling Nesprin1/Syne-1 with ab192234 at 1/50 dilution (4.3μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution; counter-stained with DAPI (blue).
      Negative controls: anti-Nesprin1/Syne-1 at 1/50 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192234).

    • Flow Cytometry - Anti-Nesprin1/Syne-1 antibody [EPR14196] - BSA and Azide free (ab240327)
      Flow Cytometry - Anti-Nesprin1/Syne-1 antibody [EPR14196] - BSA and Azide free (ab240327)

      Flow cytometric analysis of HACAT cells (paraformaldehyde-fixed, 2%) labeling Nesprin1/Syne-1 with ab192234 at 1/40 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192234).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] - BSA and Azide free (ab240327)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] - BSA and Azide free (ab240327)

      Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Nesprin1/Syne-1 with ab192234 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192234).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] - BSA and Azide free (ab240327)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] - BSA and Azide free (ab240327)

      Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling Nesprin1/Syne-1 with ab192234 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192234).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] - BSA and Azide free (ab240327)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] - BSA and Azide free (ab240327)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Nesprin1/Syne-1 with ab192234 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192234).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab240327? Please let us know so that we can cite the reference in this datasheet.

    ab240327 has not yet been referenced specifically in any publications.

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