Product nameAnti-Nesprin3 antibody
See all Nesprin3 primary antibodies
DescriptionRabbit polyclonal to Nesprin3
SpecificityThis antibody detects endogenous levels of total Nesprin3 protein.
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
A synthesized peptide derived from an internal region of human Nesprin3.
- Extracts from Jurkat cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitopespecific immunogen.
Our Abpromise guarantee covers the use of ab74261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 112 kDa.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionComponent of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system.
Sequence similaritiesBelongs to the nesprin family.
Contains 1 KASH domain.
Contains 2 spectrin repeats.
DomainThe KASH domain is involved in the binding to SUN1 and SUN2 through recognition of their SUN domains.
Cellular localizationNucleus outer membrane. Located at the rough reticulum endoplasmic (RER) in some cells.
- Information by UniProt
- C14orf49 antibody
- Chromosome 14 open reading frame 49 antibody
- FLJ25605 antibody
All lanes : Anti-Nesprin3 antibody (ab74261) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from Jurkat cells plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.
Predicted band size: 112 kDa
Observed band size: 112 kDa
ICC/IF image of ab74261 stained HeLa cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74261, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Ji Y et al. Sperm-associated antigen 4 (SPAG4) as a new cancer marker interacts with Nesprin3 to regulate cell migration in lung carcinoma. Oncol Rep 40:783-792 (2018). Read more (PubMed: 29901114) »
- Coffinier C et al. Abnormal development of the cerebral cortex and cerebellum in the setting of lamin B2 deficiency. Proc Natl Acad Sci U S A 107:5076-81 (2010). WB, ICC/IF ; Mouse . Read more (PubMed: 20145110) »