Key features and details
- Rabbit polyclonal to Nesprin3
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Nesprin3 antibody
See all Nesprin3 primary antibodies
DescriptionRabbit polyclonal to Nesprin3
SpecificityThis antibody detects endogenous levels of total Nesprin3 protein.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
A synthesized peptide derived from an internal region of human Nesprin3.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitopespecific immunogen.
Our Abpromise guarantee covers the use of ab74261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 112 kDa.|
FunctionComponent of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system.
Sequence similaritiesBelongs to the nesprin family.
Contains 1 KASH domain.
Contains 2 spectrin repeats.
DomainThe KASH domain is involved in the binding to SUN1 and SUN2 through recognition of their SUN domains.
Cellular localizationNucleus outer membrane. Located at the rough reticulum endoplasmic (RER) in some cells.
- Information by UniProt
- C14orf49 antibody
- Chromosome 14 open reading frame 49 antibody
- FLJ25605 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab74261 has been referenced in 2 publications.
- Ji Y et al. Sperm-associated antigen 4 (SPAG4) as a new cancer marker interacts with Nesprin3 to regulate cell migration in lung carcinoma. Oncol Rep 40:783-792 (2018). PubMed: 29901114
- Coffinier C et al. Abnormal development of the cerebral cortex and cerebellum in the setting of lamin B2 deficiency. Proc Natl Acad Sci U S A 107:5076-81 (2010). WB, ICC/IF ; Mouse . PubMed: 20145110